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| Registros recuperados : 9 | |
| 2. |  | SALMAN, A.; GOUPIL P.; FILGUEIRAS, H.; CHARLES, F.; LEDOIGT, G.; SALLANON, H. Controlled atmosphere and heat shock affect PAL1 and HSP90 mRNA accumulation in fresh-cut endive (Cichorium intybus L.). European Food Research and Technology, Munchen, Germany, v.227, p.721-726, 2008. DOI 10.1007/s00217-007-0778-6| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 3. | | DJIOUA, T.; CHARLES, F.; FREIRE JUNIOR, M.; FILGUEIRAS, H.; DUCAMP-COLLIN, M.-N.; SALLANON, H. Combined effects of postharvest heat treatment and chitosan coating on quality of fresh-cut mangoes (Mangifera indica L.). International Journal of Food Science & Technology, Londres, v. 45, n. 4, p. 849-855, 2010.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | DJIOUA, T.; CHARLES, F.; FREIRE JUNIOR, M.; FILGUEIRAS, H.; DUCAMP-COLLIN, M.-N.; SALLANON, H. Combined effects of postharvest heat treatment and chitosan coating on quality of fresh-cut mangoes (Mangifera indica L.). International Journal of Food Science & Technology, Londres, v. 45, n. 4, p. 849-855, 2010.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 7. | | DJIOUA, T.; CHARLES, F.; FREIRE JÚNIOR, M.; FILGUEIRAS, H. A. C.; DUCAMP-COLLIN, M.-N.; SALLANON, H. Combined effects of postharvest heat treatment and chitosan coating on quality of fresh-cut mangoes (Mangifera indica L.). In: INTERNATIONAL SYMPOSIUM ON HUMAN HEALTH EFFECTS OF FRUITS AND VEGETABLES, 3., 2009, Avignon. Abstracts. França: International Society for Horticultural Science, 2009. p. 234.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 8. | | DJIOUA, T.; CHARLES, F.; LOPEZ-LAURI, F.; FILGUEIRAS, H.; COUDRET, A.; FREIRE JUNIOR, M.; DUCAMP-COLLIN, M.-N.; SALLANON, H. Improving the storage of minimally processed mangoes (Mangifera indica L.) by hot water treatments. Postharvest Biology and Technology, Maryland Heights, v. 52, n. 2, p. 221-226, may 2009.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | DJIOUA, T.; CHARLES, F.; LOPEZ-LAURI, F.; FILGUEIRAS, H. A. C.; COUDRET, A.; FREIRE JÚNIOR, M.; DUCAMP-COLLIN, M.-N.; SALLANON, H. Improving the storage of minimally processed mangoes (Mangifera indica L.) by hot water treatments. Postharvest Biology and Technology, v. 52, n. 2, p. 221-226, 2009.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 9 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
14/11/2019 |
|
Data da última atualização: |
01/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MACEDO, T. J. S.; SANTOS, J. M. S.; BEZERRA, M. E. S.; MENEZES, V. G.; GOUVEIA, B. B.; BARBOSA, L. M. R.; LINS, T. L. B. G; MONTE, A. P. O.; BARBERINO, R. S.; BATISTA, A. M.; BARROS, V. R. P.; WISCHRAL, A.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. |
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Afiliação: |
Taís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos. |
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Título: |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
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Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Molecular and Cellular Endocrinology, v. 495, sept. 2019. |
|
DOI: |
10.1016/j.mce.2019.110506 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium. |
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Palavras-Chave: |
Desenvolvimento folicular; Imuno-histoquímica; Imunoexpressão de proteínas receptoras; Leptina; Maturação de oócitos. |
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Thesagro: |
Ovário; Ovino. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204781/1/Immunolocalization-of-leptin-and-2019.pdf
|
|
Marc: |
LEADER 02178naa a2200385 a 4500
001 2114397
005 2021-10-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.mce.2019.110506$2DOI
100 1 $aMACEDO, T. J. S.
245 $aImmunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.$h[electronic resource]
260 $c2019
520 $aThe aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.
650 $aOvário
650 $aOvino
653 $aDesenvolvimento folicular
653 $aImuno-histoquímica
653 $aImunoexpressão de proteínas receptoras
653 $aLeptina
653 $aMaturação de oócitos
700 1 $aSANTOS, J. M. S.
700 1 $aBEZERRA, M. E. S.
700 1 $aMENEZES, V. G.
700 1 $aGOUVEIA, B. B.
700 1 $aBARBOSA, L. M. R.
700 1 $aLINS, T. L. B. G
700 1 $aMONTE, A. P. O.
700 1 $aBARBERINO, R. S.
700 1 $aBATISTA, A. M.
700 1 $aBARROS, V. R. P.
700 1 $aWISCHRAL, A.
700 1 $aQUEIROZ, M. A. A.
700 1 $aARAUJO, G. G. L. de
700 1 $aMATOS, M. H. T.
773 $tMolecular and Cellular Endocrinology$gv. 495, sept. 2019.
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