02178naa a2200385 a 450000100080000000500110000800800410001902400350006010000210009524501710011626000090028752009850029665000120128165000100129365300300130365300240133365300450135765300120140265300280141470000210144270000220146370000190148570000190150470000220152370000210154570000200156670000210158670000190160770000210162670000170164770000220166470000240168670000200171077300620173021143972021-10-01       2019    bl uuuu     u00u1 u    #d7 a10.1016/j.mce.2019.1105062DOI1 aMACEDO, T. J. S.  aImmunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.h[electronic resource]  c2019  aThe aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.  aOvário  aOvino  aDesenvolvimento folicular  aImuno-histoquímica  aImunoexpressão de proteínas receptoras  aLeptina  aMaturação de oócitos1 aSANTOS, J. M. S.1 aBEZERRA, M. E. S.1 aMENEZES, V. G.1 aGOUVEIA, B. B.1 aBARBOSA, L. M. R.1 aLINS, T. L. B. G1 aMONTE, A. P. O.1 aBARBERINO, R. S.1 aBATISTA, A. M.1 aBARROS, V. R. P.1 aWISCHRAL, A.1 aQUEIROZ, M. A. A.1 aARAUJO, G. G. L. de1 aMATOS, M. H. T.  tMolecular and Cellular Endocrinologygv. 495, sept. 2019.