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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/04/2005 |
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Data da última atualização: |
01/04/2005 |
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Autoria: |
TERZANO, G. M.; ROMANO, D.; SENATORE, E. M.; DE MAURO, G.; SCATÀ, M. C.; BORGHESE, A. |
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Título: |
Recovery of oocyties by laparoscopic follicle aspiration in FSH-treated goats. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Theriogenology, v. 53, n. 1, p. 509 2000. |
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Idioma: |
Inglês |
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Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. |
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Conteúdo: |
The objective oft his preliminary study was to compare the number and quality of oocytes recovered by laparoscopic follicle aspiration from donor goats subjected to two different schedules of superovulatory treatments with a porcine gonadotropin pituitary extract (Pluset, Serono Veterinary, Italy). Sixteen multiparous Jonica goats were used in June. Estrous was synchronized by insertion of 45 mg fluorogestone acetate-impregnated intravaginal sponges (Chronogest, Intervet, Italy) for 10 d and by the injection of 3 mg of PGF2a (Prosolvin, Intervet, Italy) administered 8 d after sponge insertion. The goats were randomIy assigned to two different treatment groups (group A and B, each n=8). Group A: 250 lU pFSH, injected (sc) as a single injection 24 h before sponge removal; group B: 250 IU pFSH injected (im) 12 h apart in a decreasing dosage over 4 d (65,65,30,30,20,20, 10, 10 lU), starting 72 h before sponge removal. Prior to laparoscopy the goats were deprived of food and water for 24 and 12 h, respectively. Aspirations were performed 24 h following sponge removal; donors were sedated with (im) 0.05 mg/kg body weight of atropin sulphate (Angelini, Italy) and 0.2 mg/kg body weight of xilazine (Rompun, Bayer, Germany) followed by (iv) 4 mg/kg body weight ofketamine (Inoketam 1000, Virbac, France). A 7 mm laparoscope, a 5 fim atraumatic grasping forceps and an 18 gauge aspiration needle connected to a vacuum line (10 mL/min) were used for ovarian manipulation and follicle aspiration. Aspiration medium consisted of phosphate-buffered saline (Euroclone, Ltd, UK) supplemented with 10 IU/mL heparin, 1% antibiotic-antimycotic and 1 g/L polyvinyl alcohol (Sigma, USA). Number of observed follicles, aspirated follicles, recovered Oocytes and viable Oocytes were recorded. Data were anaIyzed by least squares anaIysis of variance using the GLM procedure (SAS/STAT User's Guide). One goat was eliminated from data anaIysis due to ovarian adhesions and difficulties in manipulation. The results are reported as mean + SEM as follows. The results suggest that a simpler superovulatory protocol can be used in goats without affecting follicle recruitment and oocyte recovery. Further research is in progress to study goat oocytes quality and maturation in vitro. MenosThe objective oft his preliminary study was to compare the number and quality of oocytes recovered by laparoscopic follicle aspiration from donor goats subjected to two different schedules of superovulatory treatments with a porcine gonadotropin pituitary extract (Pluset, Serono Veterinary, Italy). Sixteen multiparous Jonica goats were used in June. Estrous was synchronized by insertion of 45 mg fluorogestone acetate-impregnated intravaginal sponges (Chronogest, Intervet, Italy) for 10 d and by the injection of 3 mg of PGF2a (Prosolvin, Intervet, Italy) administered 8 d after sponge insertion. The goats were randomIy assigned to two different treatment groups (group A and B, each n=8). Group A: 250 lU pFSH, injected (sc) as a single injection 24 h before sponge removal; group B: 250 IU pFSH injected (im) 12 h apart in a decreasing dosage over 4 d (65,65,30,30,20,20, 10, 10 lU), starting 72 h before sponge removal. Prior to laparoscopy the goats were deprived of food and water for 24 and 12 h, respectively. Aspirations were performed 24 h following sponge removal; donors were sedated with (im) 0.05 mg/kg body weight of atropin sulphate (Angelini, Italy) and 0.2 mg/kg body weight of xilazine (Rompun, Bayer, Germany) followed by (iv) 4 mg/kg body weight ofketamine (Inoketam 1000, Virbac, France). A 7 mm laparoscope, a 5 fim atraumatic grasping forceps and an 18 gauge aspiration needle connected to a vacuum line (10 mL/min) were used for ovarian manipulation and follicle aspirat... Mostrar Tudo |
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Palavras-Chave: |
Ednocrinologia; FSH; Ovocito. |
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Thesagro: |
Caprino; Hormônio; Laparoscopia; Reprodução Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03099naa a2200277 a 4500
001 1531057
005 2005-04-01
008 2000 bl uuuu u00u1 u #d
100 1 $aTERZANO, G. M.
245 $aRecovery of oocyties by laparoscopic follicle aspiration in FSH-treated goats.
260 $c2000
500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000.
520 $aThe objective oft his preliminary study was to compare the number and quality of oocytes recovered by laparoscopic follicle aspiration from donor goats subjected to two different schedules of superovulatory treatments with a porcine gonadotropin pituitary extract (Pluset, Serono Veterinary, Italy). Sixteen multiparous Jonica goats were used in June. Estrous was synchronized by insertion of 45 mg fluorogestone acetate-impregnated intravaginal sponges (Chronogest, Intervet, Italy) for 10 d and by the injection of 3 mg of PGF2a (Prosolvin, Intervet, Italy) administered 8 d after sponge insertion. The goats were randomIy assigned to two different treatment groups (group A and B, each n=8). Group A: 250 lU pFSH, injected (sc) as a single injection 24 h before sponge removal; group B: 250 IU pFSH injected (im) 12 h apart in a decreasing dosage over 4 d (65,65,30,30,20,20, 10, 10 lU), starting 72 h before sponge removal. Prior to laparoscopy the goats were deprived of food and water for 24 and 12 h, respectively. Aspirations were performed 24 h following sponge removal; donors were sedated with (im) 0.05 mg/kg body weight of atropin sulphate (Angelini, Italy) and 0.2 mg/kg body weight of xilazine (Rompun, Bayer, Germany) followed by (iv) 4 mg/kg body weight ofketamine (Inoketam 1000, Virbac, France). A 7 mm laparoscope, a 5 fim atraumatic grasping forceps and an 18 gauge aspiration needle connected to a vacuum line (10 mL/min) were used for ovarian manipulation and follicle aspiration. Aspiration medium consisted of phosphate-buffered saline (Euroclone, Ltd, UK) supplemented with 10 IU/mL heparin, 1% antibiotic-antimycotic and 1 g/L polyvinyl alcohol (Sigma, USA). Number of observed follicles, aspirated follicles, recovered Oocytes and viable Oocytes were recorded. Data were anaIyzed by least squares anaIysis of variance using the GLM procedure (SAS/STAT User's Guide). One goat was eliminated from data anaIysis due to ovarian adhesions and difficulties in manipulation. The results are reported as mean + SEM as follows. The results suggest that a simpler superovulatory protocol can be used in goats without affecting follicle recruitment and oocyte recovery. Further research is in progress to study goat oocytes quality and maturation in vitro.
650 $aCaprino
650 $aHormônio
650 $aLaparoscopia
650 $aReprodução Animal
653 $aEdnocrinologia
653 $aFSH
653 $aOvocito
700 1 $aROMANO, D.
700 1 $aSENATORE, E. M.
700 1 $aDE MAURO, G.
700 1 $aSCATÀ, M. C.
700 1 $aBORGHESE, A.
773 $tTheriogenology$gv. 53, n. 1, p. 509 2000.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/11/2006 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
LIMA, M. A. C. de; ALVES, R. E.; TERAO, D. |
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Afiliação: |
MARIA AUXILIADORA COELHO DE LIMA, CPATSA; RICARDO ELESBAO ALVES, CNPAT; DANIEL TERAO, CPATSA. |
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Título: |
Colheita e manuseio na pós-colheita. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: OLIVEIRA, S. M. A. de; TERAO, D.; DANTAS, S. A. F.; TAVARES, S. C. C. de H. (Ed.). Patologia pós-colheita: frutas, olerícolas e ornamentais tropicais. Brasília, DF: Embrapa Informação Tecnológica, 2006. |
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Páginas: |
cap. 16, p. 411-439. |
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Idioma: |
Português |
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Conteúdo: |
Desenvolvimento da infecção; Infecção de pré-colheita; Infecção pós-colheita; Fatores que afetam o desenvolvimento da infecção; Controle de doenças pós-colheita: manejo da cultura e da colheita, estádio de maturação, pós-colheita, tratamentos físicos, tratamentos térmicos, irradiação, luz ultravioleta, tratamentos químicos, compostos do "flavour", anidrido sulfuroso, bicarbonato de sódio, outros produtos químicos, controle biológico; Armazenamento: refrigeração, ozônio, atmosferas modificadas e controlada, óxido nitroso, níveis ultrabaixos de oxigênio e anoxia; Transporte; Combinação de métodos. |
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Palavras-Chave: |
Controle de doença. |
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Thesagro: |
Colheita; Fruta; Pós-Colheita. |
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Thesaurus NAL: |
Fruits. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178402/1/Patologia-pos-colheitac.16-p.411-439.pdf
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Marc: |
LEADER 01340naa a2200217 a 4500
001 1157688
005 2018-06-08
008 2006 bl uuuu u00u1 u #d
100 1 $aLIMA, M. A. C. de
245 $aColheita e manuseio na pós-colheita.
260 $c2006
300 $acap. 16, p. 411-439.
520 $aDesenvolvimento da infecção; Infecção de pré-colheita; Infecção pós-colheita; Fatores que afetam o desenvolvimento da infecção; Controle de doenças pós-colheita: manejo da cultura e da colheita, estádio de maturação, pós-colheita, tratamentos físicos, tratamentos térmicos, irradiação, luz ultravioleta, tratamentos químicos, compostos do "flavour", anidrido sulfuroso, bicarbonato de sódio, outros produtos químicos, controle biológico; Armazenamento: refrigeração, ozônio, atmosferas modificadas e controlada, óxido nitroso, níveis ultrabaixos de oxigênio e anoxia; Transporte; Combinação de métodos.
650 $aFruits
650 $aColheita
650 $aFruta
650 $aPós-Colheita
653 $aControle de doença
700 1 $aALVES, R. E.
700 1 $aTERAO, D.
773 $tIn: OLIVEIRA, S. M. A. de; TERAO, D.; DANTAS, S. A. F.; TAVARES, S. C. C. de H. (Ed.). Patologia pós-colheita: frutas, olerícolas e ornamentais tropicais. Brasília, DF: Embrapa Informação Tecnológica, 2006.
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Embrapa Semiárido (CPATSA) |
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