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| Registros recuperados : 308 | |
| 241. |  | ANDRADE, R. V.; LOCATELLI, G.; SARAIVA, M. A. P.; SILVA, F. R.; FRAGOSO R.; GUIMARÃES, P. M.; METHA, A.; BRASILEIRO, A. C.; GOSSI-de-SÁ, M. F.; SILVA, F. R. Construção e validação de membranas de macroarranjo de dna para o estudo da interação planta-nemátoide. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 62.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 242. | | ANDRADE, R. V. de; MEHTA, A.; GUIMARÃES, P. M.; SILVA, F. R. da; GROSSI DE SÁ, M. F.; SARAIVA, M. A. de P.; FRAGOSO, R.; BRASILEIRO, A. C. M. Construção e validação de membranas de macroarranjo de DNA para o estudo da interação planta-nematóide. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 10 p. (Embrapa Recursos Genéticos e Biotecnologia. Boletim de pesquisa e desenvolvimento, 204).| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 243. | | PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. A novel soybean hairy root system for gene functional validation. Plos One, v. 18, n. 5, e0285504, 2023. Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 244. | | ARAUJO, A. C. G. de; GUIMARAES, P. M.; MOTA, A. P. Z.; GUIMARAES, L. A.; PEREIRA, B. M.; VINSON, C. C.; LACERDA, A. L.; MARTINS, A. C. Q.; BRASILEIRO, A. C. M. Overexpression of DUF538 from wild Arachis enhances plant resistance to Meloidogyne spp. Agronomy, v. 11, n. 3, 559, 2021. Na publicação: Ana Claudia Guerra Araujo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 245. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; DA SILVA, F. R.; ARAUJO, A. C. G. de; BERTIOLI, D. J.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. Identificação de genes diferencialmente expressos em plantas de Arachis magna submetidas a estresse hídrico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 023. P. 59| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 246. | | BERTIOLI, S. C. de M. L.; CAVALCANTE, U.; GOUVEA, E. G.; BALLÉN-TABORDA, C.; SHIRASAWA, K.; GUIMARAES, P. M.; JACKSON, S. A.; BERTIOLI, D. J.; MORETZSOHN, M. de C. Identification of QTLs for rust resistance in the peanut wild species arachis magna and the development of KASP markers for marker-assisted selection. G3: Genes, Genomes e Genetics, v. 5, p. 1403-1413, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 247. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; SILVA, F. R. da; ARAÚJO, A. C. G.; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. Identifying genes related to drought through transcriptional approach. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 57.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 248. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 249. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.| Biblioteca(s): Embrapa Semiárido. |
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| 250. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 251. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Semiárido. |
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| 252. | | BUROW, M. D.; SELVARAJ, M. G.; UPADHYAYA, H.; OZIAS AKINS, P.; GUO, B.; BERTIOLI, D. J.; LEAL BERTIOLI, S. C. de M.; MORETZSOHN, M. de C.; GUIMARÃES, P. M. Genomics of peanut, a major source of oil and protein. In: MOORE, P. H.; MING, R. (Ed.). Genomics of tropical crop plants. New York: Springer Sciences, 2008. (Plant genetics and genomics: crops and models, 1). p. 421-440.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 253. | | LEAL-BERTIOLI, S. C. M.; MORETZSOHN, M. C.; ROBERTS, P. A.; BALLEN-TABORDA, C.; BORBA, T. C. O.; VALDISSER, P. A.; VIANELLO, R. P.; ARAUJO, A. C. G.; GUIMARAES, P. M.; BERTIOLI, D. J. Genetic mapping of resistance to Meloidogyne arenaria in arachis stenosperma: a new source of nematode resistance for peanut. G3: Genes, Genomes, Genetics, v. 12, 2015.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 254. | | BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; MORETZSOHN, M. de C.; NIELEN, S.; ARAUJO, A. C. G. de; SANTOS, S. P.; BRASILEIRO, A. C. M.; COOK, D.; GUIMARAES, P. M. Genetic and genomic resources of peanut to enagle the use of wild relatives for crop improvement. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 39| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 255. | | GUIMARÃES, P. M.; BRASILEIRO, A. C.; SANTOS, C.; MORGANTE, C. V.; MORETZSOHN, M. C.; SILVA, F. R. da; ARAUJO, A. C. G.; SILVA, P. I. T.; VALDEZ, V.; CASTRO, R. C. S.; BERTIOLI, D. J.; LEAL BERTIOLI, S. C. M. Gene expression profile, physiological and morphological characterisation of wild Arachis spp. under drought stress. In: ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster Abstracts... Bangkok: Generation Challenge Programme, 2008. p. 26.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 256. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 257. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.| Biblioteca(s): Embrapa Semiárido. |
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| 258. | | GUIMARAES, P. M.; PROITE, K.; BERTIOLI, S. C. de M. L.; BRASILEIRO, A. C. M.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; GIMENES, M. A.; BERTIOLI, D. Functional genomics for gene discovery in Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [s.l.]: Sociedade Brasileira de Genética, 2009. p. 307.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 259. | | BERTIOLI, S. C. de M. L.; BRASILEIRO, A. C. M.; PROITE, K.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; GIMENES, M. A.; BERTIOLI, D. J.; GUIMARAES, P. M. Functional genomics and resistance gene discovery in Arachis. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 4, 2009, Mali, África. Advances in Arachis through genomics and biotechnology (AAGB-2009). Mali: International Crops Research Institute for the Semi-Arid Tropics, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 260. | | BRASILEIRO, A. C. M.; MORGANTE, C.; SANTOS, C.; PROITE, K.; MARTINS, A.; ARAUJO, A. C. G. de; SILVA, F. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Functional genomics studies on wild arachis. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 9., 2009, St. Louis, Missouri. Proceedings... [S.l.]: The International Society for Plant Molecular Biology, 2009. p.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 308 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A novel soybean hairy root system for gene functional validation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
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DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
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Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
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Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
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024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI
100 1 $aPEREIRA, B. M.
245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource]
260 $c2023
500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
650 $aSoybeans
650 $aGlycine Max
650 $aMelhoramento Genético Vegetal
650 $aMeloidogyne spp
650 $aSoja
653 $aAgrobacterium rhizogenes
653 $aTransgenic soybean
700 1 $aARRAES, F.
700 1 $aMARTINS, A. C. Q.
700 1 $aALVES, N. S. F.
700 1 $aMELO, B. P.
700 1 $aMORGANTE, C. V.
700 1 $aSARAIVA, M. A. P.
700 1 $aSA, M. F. G. de
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Embrapa Semiárido (CPATSA) |
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