02598naa a2200337 a 450000100080000000500110000800800410001902400550006010000190011524500930013426000090022750000800023652015750031665000130189165000160190465000350192065000200195565000090197565300290198465300230201370000150203670000220205170000200207370000160209370000200210970000220212970000200215170000210217170000250219277300430221721540662024-04-25       2023    bl uuuu     u00u1 u    #d7 ahttps://doi. org/10.1371/journal.pone.02855042DOI1 aPEREIRA, B. M.  aA novel soybean hairy root system for gene functional validation.h[electronic resource]  c2023  aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.  aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.  aSoybeans  aGlycine Max  aMelhoramento Genético Vegetal  aMeloidogyne spp  aSoja  aAgrobacterium rhizogenes  aTransgenic soybean1 aARRAES, F.1 aMARTINS, A. C. Q.1 aALVES, N. S. F.1 aMELO, B. P.1 aMORGANTE, C. V.1 aSARAIVA, M. A. P.1 aSA, M. F. G. de1 aGUIMARAES, P. M.1 aBRASILEIRO, A. C. M.  tPlos Onegv. 18, n. 5, e0285504, 2023.