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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Suínos e Aves. |
Data corrente: |
09/09/2021 |
Data da última atualização: |
15/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HUL, L. M.; IBELLI, A. M. G.; SAVOLDI, I. R.; MARCELINO, D. E. P.; FERNANDES, L. T.; PEIXOTO, J. O.; CANTAO, M. E.; HIGA, R. H.; GIACHETTO, P. F.; COUTINHO, L. L.; LEDUR, M. C. |
Afiliação: |
LUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA. |
Título: |
Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientific Reports, v. 11, n. 1, p. 1-13. 2021. |
DOI: |
https://doi.org/10.1038/s41598-021-97306-3 |
Idioma: |
Inglês |
Notas: |
Article number: 17965. |
Conteúdo: |
Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans. |
Palavras-Chave: |
Análises de expressão diferencial; Cartilagem da cabeça do fêmur; Differential expression analysis; Expressão gênica; Reação da cadeia de polimerase; Separação da cabeça femoral; Sequenciamento de RNA; Transcriptoma da cartilagem do fêmur. |
Thesagro: |
Frango; Frango de Corte. |
Thesaurus Nal: |
Broiler chickens; Gene expression; Quantitative polymerase chain reaction; Transcriptome. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226988/1/final9712.pdf
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Marc: |
LEADER 02920naa a2200433 a 4500 001 2135377 005 2021-10-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-021-97306-3$2DOI 100 1 $aHUL, L. M. 245 $aDifferentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.$h[electronic resource] 260 $c2021 500 $aArticle number: 17965. 520 $aLocomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans. 650 $aBroiler chickens 650 $aGene expression 650 $aQuantitative polymerase chain reaction 650 $aTranscriptome 650 $aFrango 650 $aFrango de Corte 653 $aAnálises de expressão diferencial 653 $aCartilagem da cabeça do fêmur 653 $aDifferential expression analysis 653 $aExpressão gênica 653 $aReação da cadeia de polimerase 653 $aSeparação da cabeça femoral 653 $aSequenciamento de RNA 653 $aTranscriptoma da cartilagem do fêmur 700 1 $aIBELLI, A. M. G. 700 1 $aSAVOLDI, I. R. 700 1 $aMARCELINO, D. E. P. 700 1 $aFERNANDES, L. T. 700 1 $aPEIXOTO, J. O. 700 1 $aCANTAO, M. E. 700 1 $aHIGA, R. H. 700 1 $aGIACHETTO, P. F. 700 1 $aCOUTINHO, L. L. 700 1 $aLEDUR, M. C. 773 $tScientific Reports$gv. 11, n. 1, p. 1-13. 2021.
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Embrapa Suínos e Aves (CNPSA) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
15/10/2004 |
Data da última atualização: |
06/07/2009 |
Autoria: |
CARRIJO, F. R. F.; ALBUQUERQUE, L. C.; GIORDANO, L. B.; BOITEUX, L. S.; PEREIRA, W.; NAGATA, T.; INOUE-NAGATA, A. K. |
Afiliação: |
Embrapa Hortaliças, Brasília, DF. |
Título: |
Molecular identification of begomoviruses in tomato-associated weeds and altenative host species. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Virus Reviews & Research, Florianópolis, v. 9, p. 241-242, 2004. Suplement 1. |
Idioma: |
Inglês |
Notas: |
Resumos. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004. |
Palavras-Chave: |
BGMV; Erva-de-touro; Hospedeira; Mosca-branca; Oxalis latifolia; Planta daninha; SiMoV; Xique-xique. |
Thesagro: |
Bemisia Tabaci; Erva Daninha; Lycopersicon Esculentum; Tomate; Trevo. |
Thesaurus NAL: |
Begomovirus; Crotalaria incana; Tridax procumbens. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01139naa a2200385 a 4500 001 1776809 005 2009-07-06 008 2004 bl uuuu u00u1 u #d 100 1 $aCARRIJO, F. R. F. 245 $aMolecular identification of begomoviruses in tomato-associated weeds and altenative host species. 260 $c2004 500 $aResumos. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004. 650 $aBegomovirus 650 $aCrotalaria incana 650 $aTridax procumbens 650 $aBemisia Tabaci 650 $aErva Daninha 650 $aLycopersicon Esculentum 650 $aTomate 650 $aTrevo 653 $aBGMV 653 $aErva-de-touro 653 $aHospedeira 653 $aMosca-branca 653 $aOxalis latifolia 653 $aPlanta daninha 653 $aSiMoV 653 $aXique-xique 700 1 $aALBUQUERQUE, L. C. 700 1 $aGIORDANO, L. B. 700 1 $aBOITEUX, L. S. 700 1 $aPEREIRA, W. 700 1 $aNAGATA, T. 700 1 $aINOUE-NAGATA, A. K. 773 $tVirus Reviews & Research, Florianópolis$gv. 9, p. 241-242, 2004. Suplement 1.
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