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| Registros recuperados : 114 | |
| 41. |  | NOVELLI, V. M.; KUBO, K. S.; FREITAS-ASTÚA, J.; MACHADO, M. A.; SALAROLI, R. B.; KITAJIMA, E. W. Natural infection of peace lily (Spatiphyllum wallisi) by a nuclear type of Brevipalpus-transmitted virus. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD-ROM. (E.3.1.42)| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 42. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 43. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 45. | | FREITAS-ÁSTUA, J.; FADEL, A. L.; BASTIANEL, M.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; MACHADO, M. A. Resposta diferencial de espécies e de híbridos de citros à leprose. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 809-814, jul. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 46. | | BASTIANEL, M.; PEREIRA, J. A.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; AZEVEDO, F. A.; FREITAS-ASTUA, J. Resposta de tangerinas e híbridos ao virus da leprose dos citros. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1458| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 47. | | BASTIANEL, M.; FREITAS-ÁSTUA, J.; NICOLINI, F.; SEGATTI, N.; NOVELLI, V. M.; RODRIGUES, V.; MEDINA, C. L.; MACHADO, M. A. Response of mandarin cultivars and hybrids to citrus leprosis virus. Journal of Plant Pathology, Netherlands, v. 90, n. 2, p. 305-310, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 48. | | KUBO, K. S.; STUART, R. M.; BASTIANEL, M.; COSTA, F. N.; FREITAS-ASTÚA, J.; MACHADO, M. A. Response of Murcott tangor and Pera sweet orange to Citrus leprosis virus C (CiLV-C) and Brevipalpus phoenicis mites analyzed by 2DE. In:JOINT ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS AND THE CANADIAN SOCIETY OF PLANT PHYSIOLOGISTS, 2009, Honolulu, Hawaii. Plant Biology Meeting: final program. [S.n.], 2009. P48071| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 49. | | VENÂNCIO, A. L.; MILORI, D. M. B. P.; CARDINALI, M. C. B.; FERREIRA, E. J.; FREITAS-ASTÚA, J. Detecção precoce do Greening em citros usando espectroscopia de fluorescência induzida por laser (LIFS). In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. p. 46 (Embrapa Pecuária Sudeste. Documentos, 90)| Biblioteca(s): Embrapa Instrumentação. |
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| 50. | | COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. Abstract 109| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 51. | | HALFELD-VIEIRA, B. A.; MARSARO JÚNIOR, A. L.; KITAJIMA, E. W.; PEREIRA, J. A.; FREITAS-ÁSTUA, J.; NAVIA, D. Ocorrência da leprose dos citros no Estado de Roraima. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 892.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 52. | | HALFELD-VIEIRA, B. A.; MARSARO JÚNIOR, A. L.; KITAJIMA, E. W.; PEREIRA, J. A.; FREITAS-ASTÚA, J.; NAVIA, D. Ocorrência da leprose dos citros no Estado de Roraima. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 42., 2009. Resumos... Tropical Plant Pathology, v. 34, Supl. 2009.| Biblioteca(s): Embrapa Roraima. |
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| 53. | | LUCHETA, A. R.; SILVA-PINHATI, A. C. O.; BASÍLIO-PALMIERI, A. C.; BERGER, I. J.; FREITAS-ÁSTUA, J.; CRISTOFANI, M. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 819-831, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 54. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; REIS, M. S.; TARGON, M. L. P. N.; MACHADO, M. A. In silico prediction of gene expression patterns in Citrus flavedo. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 752-760, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 58. | | PEREIRA, J. A.; ANTONIOLI - LUIZON, R.; NICOLINI, F.; LOCALI-FABRIS, E. C.; KUBO, K. S.; M ACHADO, M. A.; FREITAS-ASTÚA, J. Análise da variabilidade genética do gene da capa protéica do vírus da leprose dos citros. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., p. 287, ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 59. | | SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 60. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 114 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/03/2009 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. |
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Afiliação: |
Elliot Watanabe Kitajima, ESALQ; R. F. Calegario, ESALQ; Valdenice Moreira Novelli, APTA; Eliane Cristina Locali-Fabris, APTA; Marinês Bastianel, APTA; F. Francischini, Alellyx Genomics; Juliana Freitas-Ástua, CNPMF. |
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Título: |
In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. |
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Descrição Física: |
1 CD ROM |
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Idioma: |
Inglês |
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Conteúdo: |
Among the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies. MenosAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurr... Mostrar Tudo |
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Thesagro: |
Ácaro; Vírus. |
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Categoria do assunto: |
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Marc: |
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100 1 $aKITAJIMA, E. W.
245 $aIn situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type.$h[electronic resource]
260 $aIn: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.]$c2008
300 $c1 CD ROM
520 $aAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies.
650 $aÁcaro
650 $aVírus
700 1 $aCALEGARIO, R. F.
700 1 $aNOVELLI, V. M.
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aBASTIANEL, M.
700 1 $aFRANCISCHINI, F.
700 1 $aFREITAS-ASTUA, J.
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