03084nam a2200217 a 450000100080000000500110000800800410001910000200006024502130008026002120029330000130050552022000051865000110271865000110272970000210274070000190276170000250278070000180280570000210282370000220284416556342023-06-30       2008    bl uuuu     u00u1 u    #d1 aKITAJIMA, E. W.  aIn situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type.h[electronic resource]  aIn: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.]c2008  c1 CD ROM  aAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies.  aÁcaro  aVírus1 aCALEGARIO, R. F.1 aNOVELLI, V. M.1 aLOCALI-FABRIS, E. C.1 aBASTIANEL, M.1 aFRANCISCHINI, F.1 aFREITAS-ASTUA, J.