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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/04/2015 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
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Título: |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 6, e98758, 2014. |
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DOI: |
https://doi.org/10.1371/journal.pone.0098758 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Palavras-Chave: |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122336/1/Cnpgl-2014-PlosOne-Evaluation.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1371/journal.pone.0098758$2DOI
100 1 $aDORNELES, E. M. S.
245 $aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
653 $aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
700 1 $aSANTANA, J. A.
700 1 $aRIBEIRO, D.
700 1 $aDORELLA, F. A.
700 1 $aGUIMARAES, A. S.
700 1 $aMOAWAD, M. S.
700 1 $aSELIM, S. A.
700 1 $aGARALDI, A. L. M.
700 1 $aMIYOSHI, A.
700 1 $aRIBEIRO, M. G.
700 1 $aGOUVEIA, A. M. G.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tPlos One$gv. 9, n. 6, e98758, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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