02105naa a2200301 a 450000100080000000500110000800800410001902400540006010000230011424501200013726000090025752011920026665300630145870000190152170000160154070000190155670000210157570000180159670000170161470000220163170000160165370000190166970000220168870000160171070000210172670000160174777300400176320135042024-01-30       2014    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1371/journal.pone.00987582DOI1 aDORNELES, E. M. S.  aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.h[electronic resource]  c2014  aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.  aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)1 aSANTANA, J. A.1 aRIBEIRO, D.1 aDORELLA, F. A.1 aGUIMARAES, A. S.1 aMOAWAD, M. S.1 aSELIM, S. A.1 aGARALDI, A. L. M.1 aMIYOSHI, A.1 aRIBEIRO, M. G.1 aGOUVEIA, A. M. G.1 aAZEVEDO, V.1 aHEINEMANN, M. B.1 aLAGE, A. P.  tPlos Onegv. 9, n. 6, e98758, 2014.