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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Meio-Norte; Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
02/07/2003 |
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Data da última atualização: |
28/02/2013 |
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Autoria: |
ANDRADE, S. R. M.; GLORIA, B. A.; LABATE, M. T. V.; GOMES, D. G.; LABATE, C. A. |
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Título: |
Avaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Planaltina: Embrapa Cerrados, 2001. |
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Páginas: |
15 p. |
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Série: |
(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 4). |
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Idioma: |
Português |
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Conteúdo: |
Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao submetidos a sonificacao (de 0 ou 60 segundos) na presenca de Agrobacterium. Apos a inoculacao foram co-cultivados por 24 horas em meio liquido seguido de 48 horas em meio solido, ambos, contendo 20 m. L -1 de acetoceringona. Os explantes inoculados foram fixadas em solucao de Karnovsk para avaliacoes em microscopia optica e eletronica de varredura. As analises da microspia demostraram a presenca de rupturas na epiderme, quebras da parede celular e invasao da Agrobacterium nos tecidos subepidermicos. Os reultados demostraram que o metodo SAAT e uma tecnica viavel para a inoculcao de Agrobacterium em explantes de P. vulgaris. |
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Palavras-Chave: |
Biotecnologia: transgenico; Common bean; Genetic; Phaseolus vugaris; SAAT; Transformação genética; Transformation; Transgênico. |
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Thesagro: |
Bactéria; Biotecnologia; Cerrado; Feijão; Genética Vegetal; Infecção; Microscopia; Phaseolus Vulgaris. |
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Thesaurus Nal: |
plant genetics. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2010/23710/1/bolpd-04.pdf
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Marc: |
LEADER 02472nam a2200385 a 4500
001 1567067
005 2013-02-28
008 2001 bl uuuu u0uu1 u #d
100 1 $aANDRADE, S. R. M.
245 $aAvaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia.
260 $aPlanaltina: Embrapa Cerrados$c2001
300 $a15 p.
490 $a(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 4).
520 $aPhaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao submetidos a sonificacao (de 0 ou 60 segundos) na presenca de Agrobacterium. Apos a inoculacao foram co-cultivados por 24 horas em meio liquido seguido de 48 horas em meio solido, ambos, contendo 20 m. L -1 de acetoceringona. Os explantes inoculados foram fixadas em solucao de Karnovsk para avaliacoes em microscopia optica e eletronica de varredura. As analises da microspia demostraram a presenca de rupturas na epiderme, quebras da parede celular e invasao da Agrobacterium nos tecidos subepidermicos. Os reultados demostraram que o metodo SAAT e uma tecnica viavel para a inoculcao de Agrobacterium em explantes de P. vulgaris.
650 $aplant genetics
650 $aBactéria
650 $aBiotecnologia
650 $aCerrado
650 $aFeijão
650 $aGenética Vegetal
650 $aInfecção
650 $aMicroscopia
650 $aPhaseolus Vulgaris
653 $aBiotecnologia: transgenico
653 $aCommon bean
653 $aGenetic
653 $aPhaseolus vugaris
653 $aSAAT
653 $aTransformação genética
653 $aTransformation
653 $aTransgênico
700 1 $aGLORIA, B. A.
700 1 $aLABATE, M. T. V.
700 1 $aGOMES, D. G.
700 1 $aLABATE, C. A.
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Registro original: |
Embrapa Cerrados (CPAC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/11/2023 |
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Data da última atualização: |
16/11/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, A. R. da; SILVA, D. da C.; PINTO, K. N. dos S.; SANTOS FILHO, H. P.; COELHO FILHO, M. A.; FERREIRA, C. F.; GESTEIRA, A. da S. |
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Afiliação: |
ADIELLE RODRIGUES DA SILVA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; DELMIRA DA COSTA SILVA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; KALIANE NASCIMENTO DOS SANTOS PINTO, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; HERMES PEIXOTO SANTOS FILHO; MAURICIO ANTONIO COELHO FILHO, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Epigenetic Memory In Citrus Scion/Rootstock Combinations Formed With Buds Challenged By Phytophthora Citrophthora. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: BRAZILIAN CONGRESS OF GENETICS, 68., 2023, Ouro Preto. Paleogenomics: sequencing ancient DNA. E-book. Ribeirão Preto: Sociedade Brasileira de Genética, 2023. |
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Páginas: |
p.380 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Studies show that DNA methylation is involved in plant immunity, but little is known about how plants employ this epigenetic mechanism to adjust their responses to biotic stress, especially in plant interactions with hemibiotrophic pathogens such as Citrus Phytophthora. The objective of this work was to verify the activation of the stress memory in the responses to infection by P. citrophthora in combinations scion/rootstock of citrus - seedlings of 'Pera' sweet orange and 'Tahiti' acid lime grafted in rootstock 'Rangpur' lime and 'Tropical' sunki mandarin formed from buds challenged by this pathogen. The methodology used was the inoculation with mycelium disc of P. citrophthora on rootstock stems, and buds from the scion of these plants were grafted onto new rootstocks to verify that these new seedlings they had memorized the stress and responded better to the infection of the pathogen. Results showed that in the combinations Pera/Rangpur and Tahiti/Tropical combinations, with variety more and less sensitive to the pathogen, the seedlings formed with buds challenged by this pathogen showed smaller lesion size on the stem and more intense frequency for the complete methylation and hemimethylation profile. The opposite reaction was observed in Tahiti/Rangpur, two more sensitive varieties, and Pera/Tropical, two less sensitive varieties, which showed a higher frequency of the hemimethylation and non-methylation profile. It is concluded that the scion/rootstock interaction influenced the response to infection caused by the oomycete and DNA methylation patterns, and combinations with more and less sensitive varieties challenged with the pathogen showed an improved response to infection. MenosAbstract: Studies show that DNA methylation is involved in plant immunity, but little is known about how plants employ this epigenetic mechanism to adjust their responses to biotic stress, especially in plant interactions with hemibiotrophic pathogens such as Citrus Phytophthora. The objective of this work was to verify the activation of the stress memory in the responses to infection by P. citrophthora in combinations scion/rootstock of citrus - seedlings of 'Pera' sweet orange and 'Tahiti' acid lime grafted in rootstock 'Rangpur' lime and 'Tropical' sunki mandarin formed from buds challenged by this pathogen. The methodology used was the inoculation with mycelium disc of P. citrophthora on rootstock stems, and buds from the scion of these plants were grafted onto new rootstocks to verify that these new seedlings they had memorized the stress and responded better to the infection of the pathogen. Results showed that in the combinations Pera/Rangpur and Tahiti/Tropical combinations, with variety more and less sensitive to the pathogen, the seedlings formed with buds challenged by this pathogen showed smaller lesion size on the stem and more intense frequency for the complete methylation and hemimethylation profile. The opposite reaction was observed in Tahiti/Rangpur, two more sensitive varieties, and Pera/Tropical, two less sensitive varieties, which showed a higher frequency of the hemimethylation and non-methylation profile. It is concluded that the scion/rootstock intera... Mostrar Tudo |
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Thesagro: |
Imunidade. |
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Thesaurus NAL: |
Biotic stress; DNA methylation; Epigenetics; Immunity; Oomycetes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02621nam a2200265 a 4500
001 2158474
005 2023-11-16
008 2023 bl uuuu u00u1 u #d
100 1 $aSILVA, A. R. da
245 $aEpigenetic Memory In Citrus Scion/Rootstock Combinations Formed With Buds Challenged By Phytophthora Citrophthora.$h[electronic resource]
260 $aIn: BRAZILIAN CONGRESS OF GENETICS, 68., 2023, Ouro Preto. Paleogenomics: sequencing ancient DNA. E-book. Ribeirão Preto: Sociedade Brasileira de Genética$c2023
300 $ap.380
520 $aAbstract: Studies show that DNA methylation is involved in plant immunity, but little is known about how plants employ this epigenetic mechanism to adjust their responses to biotic stress, especially in plant interactions with hemibiotrophic pathogens such as Citrus Phytophthora. The objective of this work was to verify the activation of the stress memory in the responses to infection by P. citrophthora in combinations scion/rootstock of citrus - seedlings of 'Pera' sweet orange and 'Tahiti' acid lime grafted in rootstock 'Rangpur' lime and 'Tropical' sunki mandarin formed from buds challenged by this pathogen. The methodology used was the inoculation with mycelium disc of P. citrophthora on rootstock stems, and buds from the scion of these plants were grafted onto new rootstocks to verify that these new seedlings they had memorized the stress and responded better to the infection of the pathogen. Results showed that in the combinations Pera/Rangpur and Tahiti/Tropical combinations, with variety more and less sensitive to the pathogen, the seedlings formed with buds challenged by this pathogen showed smaller lesion size on the stem and more intense frequency for the complete methylation and hemimethylation profile. The opposite reaction was observed in Tahiti/Rangpur, two more sensitive varieties, and Pera/Tropical, two less sensitive varieties, which showed a higher frequency of the hemimethylation and non-methylation profile. It is concluded that the scion/rootstock interaction influenced the response to infection caused by the oomycete and DNA methylation patterns, and combinations with more and less sensitive varieties challenged with the pathogen showed an improved response to infection.
650 $aBiotic stress
650 $aDNA methylation
650 $aEpigenetics
650 $aImmunity
650 $aOomycetes
650 $aImunidade
700 1 $aSILVA, D. da C.
700 1 $aPINTO, K. N. dos S.
700 1 $aSANTOS FILHO, H. P.
700 1 $aCOELHO FILHO, M. A.
700 1 $aFERREIRA, C. F.
700 1 $aGESTEIRA, A. da S.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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