02621nam a2200265 a 450000100080000000500110000800800410001910000200006024501420008026001670022230000100038952017260039965000180212565000200214365000160216365000130217965000140219265000140220670000200222070000240224070000240226470000240228870000200231270000230233221584742023-11-16       2023    bl uuuu     u00u1 u    #d1 aSILVA, A. R. da  aEpigenetic Memory In Citrus Scion/Rootstock Combinations Formed With Buds Challenged By Phytophthora Citrophthora.h[electronic resource]  aIn: BRAZILIAN CONGRESS OF GENETICS, 68., 2023, Ouro Preto. Paleogenomics: sequencing ancient DNA. E-book. Ribeirão Preto: Sociedade Brasileira de Genéticac2023  ap.380  aAbstract: Studies show that DNA methylation is involved in plant immunity, but little is known about how plants employ this epigenetic mechanism to adjust their responses to biotic stress, especially in plant interactions with hemibiotrophic pathogens such as Citrus Phytophthora. The objective of this work was to verify the activation of the stress memory in the responses to infection by P. citrophthora in combinations scion/rootstock of citrus - seedlings of 'Pera' sweet orange and 'Tahiti' acid lime grafted in rootstock 'Rangpur' lime and 'Tropical' sunki mandarin formed from buds challenged by this pathogen. The methodology used was the inoculation with mycelium disc of P. citrophthora on rootstock stems, and buds from the scion of these plants were grafted onto new rootstocks to verify that these new seedlings they had memorized the stress and responded better to the infection of the pathogen. Results showed that in the combinations Pera/Rangpur and Tahiti/Tropical combinations, with variety more and less sensitive to the pathogen, the seedlings formed with buds challenged by this pathogen showed smaller lesion size on the stem and more intense frequency for the complete methylation and hemimethylation profile. The opposite reaction was observed in Tahiti/Rangpur, two more sensitive varieties, and Pera/Tropical, two less sensitive varieties, which showed a higher frequency of the hemimethylation and non-methylation profile. It is concluded that the scion/rootstock interaction influenced the response to infection caused by the oomycete and DNA methylation patterns, and combinations with more and less sensitive varieties challenged with the pathogen showed an improved response to infection.  aBiotic stress  aDNA methylation  aEpigenetics  aImmunity  aOomycetes  aImunidade1 aSILVA, D. da C.1 aPINTO, K. N. dos S.1 aSANTOS FILHO, H. P.1 aCOELHO FILHO, M. A.1 aFERREIRA, C. F.1 aGESTEIRA, A. da S.