| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Clima Temperado. |
|
Data corrente: |
31/03/2010 |
|
Data da última atualização: |
04/05/2010 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
EMYGDIO, B. M.; PORTO, M. P.; TEIXEIRA, M. C.; GUADAGNIN, J. P.; MEIRELLES, W. F.; PEREIRA, F. R.; WINKLER, J.; ACOSTA, A.; MACHADO, J. R. A.; SILVA, S. D. A.; MILLICH, M. A. |
|
Afiliação: |
BEATRIZ MARTI EMYGDIO, CPACT; M. P. PORTO; M. C. TEIXEIRA; J. P. GUADAGNIN; W. F. MEIRELLES; F. R. PEREIRA; J. WINKLER; A. ACOSTA; J. R. A. MACHADO; S. D. A. SILVA; M. A. MILLICH. |
|
Título: |
Ensaio regional de variedades de milho na Região Sul - safra 2008/09. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: REUNIÃO TÉCNICA ANUAL DO MILHO, 54.; REUNIÃO TÉCNICA ANUAL DO SORGO, 37., 2009, Pelotas. Atas e Resumos...Veranópolis: FEPAGRO, 2009. 1 CD-ROM. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Variedades. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00767naa a2200241 a 4500
001 1747473
005 2010-05-04
008 2009 bl --- 0-- u #d
100 1 $aEMYGDIO, B. M.
245 $aEnsaio regional de variedades de milho na Região Sul - safra 2008/09.
260 $c2009
653 $aVariedades
700 1 $aPORTO, M. P.
700 1 $aTEIXEIRA, M. C.
700 1 $aGUADAGNIN, J. P.
700 1 $aMEIRELLES, W. F.
700 1 $aPEREIRA, F. R.
700 1 $aWINKLER, J.
700 1 $aACOSTA, A.
700 1 $aMACHADO, J. R. A.
700 1 $aSILVA, S. D. A.
700 1 $aMILLICH, M. A.
773 $tIn: REUNIÃO TÉCNICA ANUAL DO MILHO, 54.; REUNIÃO TÉCNICA ANUAL DO SORGO, 37., 2009, Pelotas. Atas e Resumos...Veranópolis: FEPAGRO, 2009. 1 CD-ROM.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
24/04/2021 |
|
Data da última atualização: |
12/07/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
|
Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
|
Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
|
ISSN: |
1874-3919 |
|
DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
|
Idioma: |
Inglês
Português |
|
Notas: |
Na publicação: Angela Mehta. |
|
Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
|
Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
|
Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
|
Thesaurus NAL: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
|
Categoria do assunto: |
--
S Ciências Biológicas |
|
Marc: |
LEADER 03484naa a2200469 a 4500
001 2131497
005 2021-07-12
008 2021 bl uuuu u00u1 u #d
022 $a1874-3919
024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Angela Mehta.
520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance.
650 $ablast disease
650 $adisease resistance
650 $afoliar diseases
650 $aMagnaporthe oryzae
650 $aplant diseases and disorders
650 $aproteomics
650 $arice
650 $aArroz
650 $aBrusone
650 $aDoença de Planta
650 $aOryza Sativa
653 $aGene ontology
653 $aPlant immunity
653 $aPTO-based TIGS
653 $aRT-qPCR
653 $aS-gene
653 $aTransient-Induced gene silencing
700 1 $aBEVITORI, R.
700 1 $aMELLO, R. N. de
700 1 $aCINTRA, M. M. D. F.
700 1 $aOLIVEIRA NETO, O. B.
700 1 $aFONTES, W.
700 1 $aCASTRO, M. S.
700 1 $aSOUSA, M. V.
700 1 $aFRANCO, O. L.
700 1 $aREIS, A. M. dos
773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
