03484naa a2200469 a 450000100080000000500110000800800410001902200140006002400530007410000250012724501700015226000090032250000350033152021020036665000180246865000230248665000200250965000230252965000330255265000150258565000090260065000100260965000120261965000220263165000170265365300180267065300190268865300190270765300120272665300110273865300370274970000170278670000200280370000240282370000250284770000150287270000180288770000170290570000180292270000200294077300540296021314972021-07-12       2021    bl uuuu     u00u1 u    #d  a1874-39197 ahttps://doi.org/10.1016/j.jprot.2021.1042232DOI1 aTÁVORA, F. T. P. K.  aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.h[electronic resource]  c2021  aNa publicação: Angela Mehta.  aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance.  ablast disease  adisease resistance  afoliar diseases  aMagnaporthe oryzae  aplant diseases and disorders  aproteomics  arice  aArroz  aBrusone  aDoença de Planta  aOryza Sativa  aGene ontology  aPlant immunity  aPTO-based TIGS  aRT-qPCR  aS-gene  aTransient-Induced gene silencing1 aBEVITORI, R.1 aMELLO, R. N. de1 aCINTRA, M. M. D. F.1 aOLIVEIRA NETO, O. B.1 aFONTES, W.1 aCASTRO, M. S.1 aSOUSA, M. V.1 aFRANCO, O. L.1 aREIS, A. M. dos  tJournal of Proteomicsgv. 241, 104223, June 2021.