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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
16/04/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERNANDES, C. A. de C.; PEREIRA, J. R.; SOUZA, V. O.; FIGUEIREDO, A. C. S. de; VIANA, J. H. M.; SIQUEIRA, L. G. B.; PALHAO, M. P. |
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Afiliação: |
CARLOS ANTONIO DE CARVALHO FERNANDES, UNIVERSIDADE JOSE DO ROSARIO VELLANO; JESSICA RUIZ PEREIRA, UNIVERSIDADE JOSE DO ROSARIO VELLANO; VINÍCIUS OLIVEIRA SOUZA, UNIVERSIDADE JOSE DO ROSARIO VELLANO; ANA CRISTINA SILVA DE FIGUEIREDO, UNIVERSIDADE JOSE DO ROSARIO VELLANO; JOAO HENRIQUE MOREIRA VIANA, Cenargen; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MILLER PEREIRA PALHAO, UNIVERSIDADE JOSE DO ROSARIO VELLANO. |
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Título: |
Timing of early resynchronization protocols affects subsequent pregnancy outcome in dairy cows. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 167, p. 61-66, 2021. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.03.009 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the outcomes of an early resynchronization protocol (Resynch)initiated at different timepoints after timed artificial insemination (TAI) and with unknown pregnancystatus. Holstein cows (n¼164) were submitted to the following TAI protocol: D0, insertion of anintravaginal progesterone (P4) device and 2 mg im estradiol benzoate (EB); D8, removal of P4 device andtreatment with 0.5 mg im sodium cloprostenol (PGF); D9, 0.1 mg im Lecirelin (LEC); and D10, TAI1. Cowswere then randomly assigned to Resynch protocols starting either on day 20 (Resynch20D,n¼82) or 25after TAI1 (Resynch25D,n¼82) with the insertion of a new P4 device and EB treatment. In both groups,P4 device was removed on day 8 after the beginning of Resynch, the same day of pregnancy diagnosis byultrasonography. In pregnant cows there was no further action. Non-pregnant cows were treated with0.5 mg im PGF, had a blood sample collected for serum P4 analysis and we measured and recorded thesize of the largest follicle and the presence or absence of a corpus luteum (CL). One day later, cows weretreated with 0.1 mg im LEC and TAI2 occurred 12e14 h later. The diameter of the largest follicle andserum P4 were compared between groups by ANOVA for the main effects of treatment, presence of a CL,and their interaction, whereas pregnancy per artificial insemination (P/AI) and the percentage of cowswith a CL on the day of ultrasonography were analyzed using the Chi-square test. Follicle diameter on day8 of Resynch was greater for cows in the Resynch20D group compared with Resynch25D (15.9±3.9 vs12.2±2.5 mm, respectively; P¼0.046). The Resynch25D group had a greater percentage of cows with aCL (51.9 vs 18.9%, respectively; P¼0.0008) and higher serum P4 (2.8±1.1 vs 1.7±0.8 ng/mL; P¼0.041)at the end of the protocol compared with Resynch20D. P/AI at TAI1 was 35.4 and 36.6% (P>0.10) forcows enrolled in Resynch20D and Resynch25D groups, respectively. P/AI to TAI2, after Resynch protocols,was greater in Resynch25D than Resynch20D (44.2 vs 22.6%, respectively; P<0.05). In conclusion,starting an early resynchronization protocol 25 days after TAI increases P/AI compared with starting 20days after TAI, and this was associated with a presumed greater proportion of cows with a functional CLat the moment of P4 device removal. MenosThe aim of this study was to evaluate the outcomes of an early resynchronization protocol (Resynch)initiated at different timepoints after timed artificial insemination (TAI) and with unknown pregnancystatus. Holstein cows (n¼164) were submitted to the following TAI protocol: D0, insertion of anintravaginal progesterone (P4) device and 2 mg im estradiol benzoate (EB); D8, removal of P4 device andtreatment with 0.5 mg im sodium cloprostenol (PGF); D9, 0.1 mg im Lecirelin (LEC); and D10, TAI1. Cowswere then randomly assigned to Resynch protocols starting either on day 20 (Resynch20D,n¼82) or 25after TAI1 (Resynch25D,n¼82) with the insertion of a new P4 device and EB treatment. In both groups,P4 device was removed on day 8 after the beginning of Resynch, the same day of pregnancy diagnosis byultrasonography. In pregnant cows there was no further action. Non-pregnant cows were treated with0.5 mg im PGF, had a blood sample collected for serum P4 analysis and we measured and recorded thesize of the largest follicle and the presence or absence of a corpus luteum (CL). One day later, cows weretreated with 0.1 mg im LEC and TAI2 occurred 12e14 h later. The diameter of the largest follicle andserum P4 were compared between groups by ANOVA for the main effects of treatment, presence of a CL,and their interaction, whereas pregnancy per artificial insemination (P/AI) and the percentage of cowswith a CL on the day of ultrasonography were analyzed using the Chi-square test. Follicle diamet... Mostrar Tudo |
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Palavras-Chave: |
Bovine; TAI protocol. |
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Thesagro: |
Gado Leiteiro; Inseminação Artificial; Reprodução Animal. |
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Thesaurus Nal: |
Artificial insemination; Reproductive efficiency. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03248naa a2200289 a 4500
001 2133324
005 2021-08-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.03.009$2DOI
100 1 $aFERNANDES, C. A. de C.
245 $aTiming of early resynchronization protocols affects subsequent pregnancy outcome in dairy cows.$h[electronic resource]
260 $c2021
520 $aThe aim of this study was to evaluate the outcomes of an early resynchronization protocol (Resynch)initiated at different timepoints after timed artificial insemination (TAI) and with unknown pregnancystatus. Holstein cows (n¼164) were submitted to the following TAI protocol: D0, insertion of anintravaginal progesterone (P4) device and 2 mg im estradiol benzoate (EB); D8, removal of P4 device andtreatment with 0.5 mg im sodium cloprostenol (PGF); D9, 0.1 mg im Lecirelin (LEC); and D10, TAI1. Cowswere then randomly assigned to Resynch protocols starting either on day 20 (Resynch20D,n¼82) or 25after TAI1 (Resynch25D,n¼82) with the insertion of a new P4 device and EB treatment. In both groups,P4 device was removed on day 8 after the beginning of Resynch, the same day of pregnancy diagnosis byultrasonography. In pregnant cows there was no further action. Non-pregnant cows were treated with0.5 mg im PGF, had a blood sample collected for serum P4 analysis and we measured and recorded thesize of the largest follicle and the presence or absence of a corpus luteum (CL). One day later, cows weretreated with 0.1 mg im LEC and TAI2 occurred 12e14 h later. The diameter of the largest follicle andserum P4 were compared between groups by ANOVA for the main effects of treatment, presence of a CL,and their interaction, whereas pregnancy per artificial insemination (P/AI) and the percentage of cowswith a CL on the day of ultrasonography were analyzed using the Chi-square test. Follicle diameter on day8 of Resynch was greater for cows in the Resynch20D group compared with Resynch25D (15.9±3.9 vs12.2±2.5 mm, respectively; P¼0.046). The Resynch25D group had a greater percentage of cows with aCL (51.9 vs 18.9%, respectively; P¼0.0008) and higher serum P4 (2.8±1.1 vs 1.7±0.8 ng/mL; P¼0.041)at the end of the protocol compared with Resynch20D. P/AI at TAI1 was 35.4 and 36.6% (P>0.10) forcows enrolled in Resynch20D and Resynch25D groups, respectively. P/AI to TAI2, after Resynch protocols,was greater in Resynch25D than Resynch20D (44.2 vs 22.6%, respectively; P<0.05). In conclusion,starting an early resynchronization protocol 25 days after TAI increases P/AI compared with starting 20days after TAI, and this was associated with a presumed greater proportion of cows with a functional CLat the moment of P4 device removal.
650 $aArtificial insemination
650 $aReproductive efficiency
650 $aGado Leiteiro
650 $aInseminação Artificial
650 $aReprodução Animal
653 $aBovine
653 $aTAI protocol
700 1 $aPEREIRA, J. R.
700 1 $aSOUZA, V. O.
700 1 $aFIGUEIREDO, A. C. S. de
700 1 $aVIANA, J. H. M.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aPALHAO, M. P.
773 $tTheriogenology$gv. 167, p. 61-66, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/05/2024 |
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Data da última atualização: |
13/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
DIAS, I. A.; HORTA, R. P.; MATOS, M.; HELM, C. V.; MAGALHAES, W. L. E.; LIMA, E. A. de; SILVA, B. J. G. da; MUNIZ, G. I. B. de; CADEMARTORI, P. H. G. de. |
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Afiliação: |
IVANA AMORIM DIAS, UNIVERSIDADE FEDERAL DO PARANÁ; RAYTA PAIM HORTA, UNIVERSIDADE FEDERAL DO PARANÁ; MAILSON MATOS, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; EDSON ALVES DE LIMA, CNPF; BRUNO JOSÉ GONÇALVES DA SILVA, UNIVERSIDADE FEDERAL DO PARANÁ; GRACIELA INES BOLZON DE MUNIZ, UNIVERSIDADE FEDERAL DO PARANÁ; PEDRO HENRIQUE GONZALEZ DE CADEMARTORI, UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Exploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefinery, p. 1-12, 2023. |
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DOI: |
http://dx.doi.org/10.1007/s13399-023-04561-7 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
Improving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities. MenosImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal act... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial potential; Aqueous fraction; Levoglucosan; Storage stability. |
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Thesaurus NAL: |
Antioxidant activity; Phenolic compounds. |
|
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 03046naa a2200313 a 4500
001 2164243
005 2024-05-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1007/s13399-023-04561-7$2DOI
100 1 $aDIAS, I. A.
245 $aExploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil.$h[electronic resource]
260 $c2023
500 $aOnline first.
520 $aImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities.
650 $aAntioxidant activity
650 $aPhenolic compounds
653 $aAntimicrobial potential
653 $aAqueous fraction
653 $aLevoglucosan
653 $aStorage stability
700 1 $aHORTA, R. P.
700 1 $aMATOS, M.
700 1 $aHELM, C. V.
700 1 $aMAGALHAES, W. L. E.
700 1 $aLIMA, E. A. de
700 1 $aSILVA, B. J. G. da
700 1 $aMUNIZ, G. I. B. de
700 1 $aCADEMARTORI, P. H. G. de
773 $tBiomass Conversion and Biorefinery, p. 1-12, 2023.
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