03030naa a2200301 a 450000100080000000500110000800800410001902400540006010000160011424501800013026000090031052020390031965000250235865000230238365300280240665300210243465300170245565300220247270000170249470000140251170000160252570000240254170000190256570000230258470000230260770000290263077300690265921642432024-12-11       2024    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1007/s13399-023-04561-72DOI1 aDIAS, I. A.  aExploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil.h[electronic resource]  c2024  aImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities.  aAntioxidant activity  aPhenolic compounds  aAntimicrobial potential  aAqueous fraction  aLevoglucosan  aStorage stability1 aHORTA, R. P.1 aMATOS, M.1 aHELM, C. V.1 aMAGALHAES, W. L. E.1 aLIMA, E. A. de1 aSILVA, B. J. G. da1 aMUNIZ, G. I. B. de1 aCADEMARTORI, P. H. G. de  tBiomass Conversion and Biorefinerygv. 14, p. 24333-24344, 2024.