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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/11/2010 |
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Data da última atualização: |
05/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PASQUAL, M.; PIO, L. A. S; SILVA, S. de O. e; DAVIDE, L. C; SEREJO, J. A. dos S.; ROCHA, H. S. |
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Afiliação: |
MOACIR PASQUAL, UFLA; LEILA APARECIDA SALLES PIO, UFLA; SEBASTIÃO DE OLIVEIRA E SILVA; LISETE CHAMMA DAVIDE, UFLA; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; HERMINIO SOUZA ROCHA, CNPMF. |
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Título: |
Chromosome doubling in musa acuminata colla. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM. |
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Volume: |
v.2 |
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Páginas: |
p. 757. |
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Idioma: |
Inglês |
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Notas: |
PDF. S18.201. |
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Conteúdo: |
The aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible. MenosThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 ... Mostrar Tudo |
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Palavras-Chave: |
Cultivares. |
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Thesagro: |
Banana. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02437naa a2200241 a 4500
001 1866599
005 2011-04-05
008 2010 bl uuuu u00u1 u #d
100 1 $aPASQUAL, M.
245 $aChromosome doubling in musa acuminata colla.
260 $c2010
300 $ap. 757. v.2
490 $vv.2
500 $aPDF. S18.201.
520 $aThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible.
650 $aBanana
653 $aCultivares
700 1 $aPIO, L. A. S
700 1 $aSILVA, S. de O. e
700 1 $aDAVIDE, L. C
700 1 $aSEREJO, J. A. dos S.
700 1 $aROCHA, H. S.
773 $tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/06/2016 |
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Data da última atualização: |
08/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BORTOLOTTO, O. C.; BUENO, A. F.; STOPA, Y. K.; SILVA, G. V.; QUEIROZ, A. P. |
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Afiliação: |
ORCIAL C. BORTOLOTTO, UFPR; ADENEY DE FREITAS BUENO, CNPSO; YNAIARA K. STOPA, UENP - Cornélio Procópio; GABRIELA V. SILVA, UEL; ANA PAULA QUEIROZ, IAC. |
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Título: |
Development of Dichelops melacanthus and its egg parasitoid Telenomus podisi reared on Bt-soybean MON 87701 x MON 89788 and its near conventional isoline under different temperatures. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 88, n. 2, p. 1023-1034, 2016. |
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ISSN: |
1678-2690 |
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DOI: |
10.1590/0001-3765201620150257 |
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Idioma: |
Inglês |
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Conteúdo: |
Dichelops melacanthus was studied under controlled conditions (60 ± 10% RH and 14/10 h L/D photoperiod), and three constant temperatures (19, 25, and 31 ± 2 °C). Fresh pods of MON 87701 × MON 89788 soybeans and its near non-Bt isoline (A5547) were supplied to nymphs and adults. The biology of T. podisi was studied in the same controlled RH conditions, but only at the standard temperature of 25 ± 2°C. Overall, the development of D. melacanthus was better at higher temperatures, which accelerated the development of the stink bug without affecting adult biological parameters. No influence of Bt-soybeans on the biology of the pest was observed in any temperature studied, which shows that D. melacanthus is not affected by this transgenic soybean. The egg parasitoid T. podisi also was not harmed when it parasitized eggs of the pest fed with MON 87701 × MON 89788 soybeans, with similar results to those obtained in non-Bt isogenic soybeans. Thus, this study demonstrates that D. melacanthus is favored at high temperatures (31 ± 2 °C), and that neither did MON 87701 × MON 89788 soybean pods affect the development of the pest nor its parasitoid T. podisi. |
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Thesagro: |
Entomologia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157323/1/0001-3765-aabc-201620150257.pdf
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Marc: |
LEADER 01920naa a2200205 a 4500
001 2046044
005 2017-06-08
008 2016 bl uuuu u00u1 u #d
022 $a1678-2690
024 7 $a10.1590/0001-3765201620150257$2DOI
100 1 $aBORTOLOTTO, O. C.
245 $aDevelopment of Dichelops melacanthus and its egg parasitoid Telenomus podisi reared on Bt-soybean MON 87701 x MON 89788 and its near conventional isoline under different temperatures.$h[electronic resource]
260 $c2016
520 $aDichelops melacanthus was studied under controlled conditions (60 ± 10% RH and 14/10 h L/D photoperiod), and three constant temperatures (19, 25, and 31 ± 2 °C). Fresh pods of MON 87701 × MON 89788 soybeans and its near non-Bt isoline (A5547) were supplied to nymphs and adults. The biology of T. podisi was studied in the same controlled RH conditions, but only at the standard temperature of 25 ± 2°C. Overall, the development of D. melacanthus was better at higher temperatures, which accelerated the development of the stink bug without affecting adult biological parameters. No influence of Bt-soybeans on the biology of the pest was observed in any temperature studied, which shows that D. melacanthus is not affected by this transgenic soybean. The egg parasitoid T. podisi also was not harmed when it parasitized eggs of the pest fed with MON 87701 × MON 89788 soybeans, with similar results to those obtained in non-Bt isogenic soybeans. Thus, this study demonstrates that D. melacanthus is favored at high temperatures (31 ± 2 °C), and that neither did MON 87701 × MON 89788 soybean pods affect the development of the pest nor its parasitoid T. podisi.
650 $aEntomologia
700 1 $aBUENO, A. F.
700 1 $aSTOPA, Y. K.
700 1 $aSILVA, G. V.
700 1 $aQUEIROZ, A. P.
773 $tAnais da Academia Brasileira de Ciências, Rio de Janeiro$gv. 88, n. 2, p. 1023-1034, 2016.
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Embrapa Soja (CNPSO) |
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