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| Registros recuperados : 10 | |
| 1. |  | TREML, D.; VENTURELLI, G. L.; BROD, F. C. A.; FARIA, J. C.; ARISI, A. C. M. Development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris). Journal of Agricultural and Food Chemistry, Easton, v. 62, n. 49, p. 11994-12000, Dec. 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 3. | | BROD, F. C. A.; DINON, A. Z.; KOLLING, D. J.; FARIA, J. C.; ARISI, A. C. M. Development of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1. Journal of Agricultural and Food Chemistry, Easton, v. 61, n. 20, p. 4921-4926, May 2013.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 4. | | BROD, F. C. A.; PELISSER, M. R.; BERTOLDO, J. B.; VERNAL, J.; BLOCH JUNIOR, C.; TERENZI, H.; ARISI, A. C. M.; ARISI, A. C. M. Heterologous Expression and Purification of a Heat-Tolerant Staphylococcus xylosus Lipase. Molecular Biotechnology, v.44, n.2, p. 110-119, 2010| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | SAWITZKI, M. C.; FIORENTINI, A. M.; BROD, F. C. A.; TAGLIARI, C.; BERTOL, T. M.; ARISI, A. C. M.; SANT'ANNA, E. S. Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages. Brazilian Journal of Microbiology, v.38, n3. p. 547-552, 2007. Projeto n. 16.00.30001-16.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | FIORENTINI, A. M.; SAWITZKI, M. C.; BERTOL, T. M.; BROD, F. C. A.; PELISSER, M. R.; ARISI, A. C. M.; SANT'ANNA, E. S. Phenotypic and molecular characterization of staphylococcus xylosus: technological potential for use in fermented sausage Brazilian Archives of Biology and Technology, Curitiba, v. 52, n.3, p. 737-746. May/June 2009. Disponível em: . Acesso em: 09 fev. 2010 Projeto/Plano da Ação: 01.06.60.106-07| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; OLIVEIRA, E. M. M.; FARIA, J. C.; ARISI, A. C. M. Primers and probes development for specific PCR detection of genetically modified common bean (Phaseolus vulgaris) Embrapa 5.1. Journal of Agricultural and Food Chemistry, Easton, v. 60, n. 18, p. 4672-4677, May 2012.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Arroz e Feijão. |
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| 8. | | DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; SILVEIRA, J. C. M.; KOLLING, D. J.; TREML, D.; FARIA, J. C.; ARISI, A. C. M. Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | FONGARO, G.; RIBEIRO, J. B.; BERTOL, T. M.; FIRENTINI, A. M.; SAWITKI, M. C.; PEIXOTO, J. de O.; BROD, F. C. A.; ARISI, A. C. M. Isolamento de um fragmento do gene de lipase de staphylococcus xylosus U5 e AD1. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA/UNC, 4., 2010, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves, 2010. 1 CD-ROM. 4 JINC. Projeto/Plano de Ação: 03.08.06.009-02.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 10. | | VENTURELLI, G. L.; BROD, F. C. A.; ROSSI, G. B.; ZIMMERMANN, N. F.; OLIVEIRA, J. P.; FARIA, J. C.; ARISI, A. C. M. A specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene. Molecular Biotechnology, Totowa, v. 56, n. 11, p. 1060-1068, Nov. 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 10 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
09/08/2013 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BROD, F. C. A.; DINON, A. Z.; KOLLING, D. J.; FARIA, J. C.; ARISI, A. C. M. |
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Afiliação: |
FÁBIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ANDRÉIA Z. DINON, UNIVERSIDADE FEDERAL DE SANTA CATARINA; DEISE J. KOLLING, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Development of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Easton, v. 61, n. 20, p. 4921-4926, May 2013. |
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DOI: |
dx.doi.org/10.1021/jf400928k |
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Idioma: |
Inglês |
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Conteúdo: |
The genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation. |
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Thesagro: |
Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Plasmideo. |
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Thesaurus NAL: |
Beans; Genetically modified organisms; Plasmids; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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100 1 $aBROD, F. C. A.
245 $aDevelopment of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1.$h[electronic resource]
260 $c2013
520 $aThe genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation.
650 $aBeans
650 $aGenetically modified organisms
650 $aPlasmids
650 $aTransgenic plants
650 $aBiotecnologia
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPlasmideo
700 1 $aDINON, A. Z.
700 1 $aKOLLING, D. J.
700 1 $aFARIA, J. C.
700 1 $aARISI, A. C. M.
773 $tJournal of Agricultural and Food Chemistry, Easton$gv. 61, n. 20, p. 4921-4926, May 2013.
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