01833naa a2200289 a 450000100080000000500110000800800410001902400380006010000190009824501450011726000090026252009290027165000100120065000350121065000130124565000220125865000180128065000250129865000120132365000270133565000140136270000170137670000190139370000170141270000200142977300940144919636602017-11-13       2013    bl uuuu     u00u1 u    #d7 adx.doi.org/10.1021/jf400928k2DOI1 aBROD, F. C. A.  aDevelopment of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1.h[electronic resource]  c2013  aThe genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation.  aBeans  aGenetically modified organisms  aPlasmids  aTransgenic plants  aBiotecnologia  aEngenharia genética  aFeijão  aOrganismo transgênico  aPlasmideo1 aDINON, A. Z.1 aKOLLING, D. J.1 aFARIA, J. C.1 aARISI, A. C. M.  tJournal of Agricultural and Food Chemistry, Eastongv. 61, n. 20, p. 4921-4926, May 2013.