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| Registros recuperados : 10 | |
| 3. |  | FERREIRA, I. M.; VIEIRA, G. S.; BRAGA, T. F.; SILVA, T. C. F.; FRANCO, M. M.; ANTUNES, R. C. Allelic and phenotypic frequencies of point mutations (SNPs) in the HFAB, DEGAT1, LEPR, MC4R, SREBF1 and GH genes in Large White matrices. In: BRAZILIAN INTERNATIONAL CONGRESS OF GENETICS, 2017, Águas de Lindóia, 12 a 15 set. 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | BRAGA, T. F.; MARQUES, M. G.; SOUZA, A. P.; ALBRING, D.; BORGES, N. A.; DODE, M. A. N.; FRANCO, M. M. Characterization of gene expression profile in immature and in vitro matured pig oocytes. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | LEME, L. de O.; DUFORT, I.; SPRICIGO, J. F. W.; BRAGA, T. F.; SIRARD, M.; FRANCO, M. M.; DODE, M. A. N. Effect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos. Theriogenology, v. 85, 724-733, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | FERREIRA, I. M.; BRAGA, T. F.; GUIMARÃES, E. C.; DURVAL, M. C.; MENDES, L. B.; SOARES, J. S.; MATARIM, D. L.; KRUGER, B. C.; FRANCO, M. M.; ANTUNES, R. C. A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs. Genetics and Molecular Research, v. 18, n. 2, article gmr18257, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M. Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation. Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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| 10. | | BRAGA, T. F.; SILVA, T. C. F.; MARQUES, M. G.; SOUZA, A. P. de; ALBRING, D.; SILVA, L. P.; CAETANO, A. R.; DODE, M. A. N.; FRANCO, M. M. The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows. Reproduction in Domestic Animals, v. 54, n. 9, p. 1217-1229, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 10 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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Data corrente: |
23/05/2023 |
|
Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M. |
|
Afiliação: |
THAINARA C. F. SILVA, Universidade Federal de Uberlândia; MARGOT ALVES NUNES DODE, Cenargen; THIAGO F. BRAGA, Centro Universitário do Cerrado; MARIANA GROKE MARQUES, CNPSA; LUNA NASCIMENTO VARGAS, Universidade Federal de Uberlândia; OTÁVIO AUGUSTO COSTA DE FARIA, Universidade de Brasília; ANDRESSA P. DE SOUZA, Universidade do Estado de Santa Catarina; DANIELA ALBRING, Universidade do Contestado; ALEXANDRE RODRIGUES CAETANO, Cenargen; MAURICIO MACHAIM FRANCO, Cenargen. |
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Título: |
Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023. |
|
DOI: |
https://doi.org/10.1002/mrd.23685 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in
vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated
transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD)
and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript
levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were
analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the
gilt group, only FASN expression increased, while NR3C1 expression decreased after
IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow
oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1
methylation patterns exhibited a demethylation pattern in CCs and oocytes from
gilts and sows and showed statistical differences between groups. CCs from sows
had a better capacity to change transcription levels of the major genes involved in
lipid metabolism during IVM than CCs from gilts. This difference may be involved
in accumulation of lipids, acquisition of competence, and maturation of enclosed
oocytes. Our results contribute to a better understanding of mechanisms involved in
lipid metabolism and acquisition of competence in porcine COCs. |
|
Palavras-Chave: |
Donor age; Embryo production; Expressão gênica; Fatty acid; Idade do doador; Pig; Produção de embriões. |
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Thesagro: |
Ácido Graxo; Embrião Animal; Reprodução Animal; Suinocultura. |
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Thesaurus NAL: |
Gene expression. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02474naa a2200385 a 4500
001 2153952
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008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/mrd.23685$2DOI
100 1 $aSILVA, T. C. F.
245 $aCumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation.$h[electronic resource]
260 $c2023
520 $aAbstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD) and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the gilt group, only FASN expression increased, while NR3C1 expression decreased after IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1 methylation patterns exhibited a demethylation pattern in CCs and oocytes from gilts and sows and showed statistical differences between groups. CCs from sows had a better capacity to change transcription levels of the major genes involved in lipid metabolism during IVM than CCs from gilts. This difference may be involved in accumulation of lipids, acquisition of competence, and maturation of enclosed oocytes. Our results contribute to a better understanding of mechanisms involved in lipid metabolism and acquisition of competence in porcine COCs.
650 $aGene expression
650 $aÁcido Graxo
650 $aEmbrião Animal
650 $aReprodução Animal
650 $aSuinocultura
653 $aDonor age
653 $aEmbryo production
653 $aExpressão gênica
653 $aFatty acid
653 $aIdade do doador
653 $aPig
653 $aProdução de embriões
700 1 $aDODE, M. A. N.
700 1 $aBRAGA, T. F.
700 1 $aMARQUES, M. G.
700 1 $aVARGAS, L. N.
700 1 $aFARIA, O. A. C. de
700 1 $aSOUZA, A. P. de
700 1 $aALBRING, D.
700 1 $aCAETANO, A. R.
700 1 $aFRANCO, M. M.
773 $tMolecular Reproduction & Development$gv. 90, n. 5, p. 323-335. 2023.
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