02474naa a2200385 a 450000100080000000500110000800800410001902400430006010000200010324501870012326000090031052012990031965000200161865000170163865000200165565000240167565000170169965300140171665300220173065300230175265300150177565300200179065300080181065300280181870000190184670000170186570000190188270000180190170000230191970000200194270000160196270000190197870000180199777300730201521539522023-06-12       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1002/mrd.236852DOI1 aSILVA, T. C. F.  aCumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation.h[electronic resource]  c2023  aAbstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD) and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the gilt group, only FASN expression increased, while NR3C1 expression decreased after IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1 methylation patterns exhibited a demethylation pattern in CCs and oocytes from gilts and sows and showed statistical differences between groups. CCs from sows had a better capacity to change transcription levels of the major genes involved in lipid metabolism during IVM than CCs from gilts. This difference may be involved in accumulation of lipids, acquisition of competence, and maturation of enclosed oocytes. Our results contribute to a better understanding of mechanisms involved in lipid metabolism and acquisition of competence in porcine COCs.  aGene expression  aÁcido Graxo  aEmbrião Animal  aReprodução Animal  aSuinocultura  aDonor age  aEmbryo production  aExpressão gênica  aFatty acid  aIdade do doador  aPig  aProdução de embriões1 aDODE, M. A. N.1 aBRAGA, T. F.1 aMARQUES, M. G.1 aVARGAS, L. N.1 aFARIA, O. A. C. de1 aSOUZA, A. P. de1 aALBRING, D.1 aCAETANO, A. R.1 aFRANCO, M. M.  tMolecular Reproduction & Developmentgv. 90, n. 5, p. 323-335. 2023.