| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
11/01/2019 |
|
Data da última atualização: |
11/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MATTOS, B. B.; MONTEBIANCO, C.; ROMANEL, E.; SILVA, T. da F.; BERNABÉ, R. B.; SIMAS-TOSIN, F.; SOUZA, L. M.; SASSAKI, G. L.; VASLIN, M. F. S.; BARRETO-BERGTER, E. |
|
Afiliação: |
BIANCA BRAZ MATTOS, CNPS; CAROLINE MONTEBIANCO, UFRJ; ELISSON ROMANEL, UFRJ/USP; TATIANE DA FRANCA SILVA, UFRJ/USP; RENATO BARROSO BERNABÉ, UFRJ; FERNANDA SIMAS-TOSIN, UFPR; LAURO M. SOUZA, UFPR; GUILHERME L. SASSAKI, UFPR; MAITE F. S. VASLIN, UFRJ; ELIANA BARRETO-BERGTER, UFRJ. |
|
Título: |
A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 126, p. 206-216, May 2018. |
|
DOI: |
https://doi.org/10.1016/j.plaphy.2018.02.023 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively. MenosCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), a... Mostrar Tudo |
|
Palavras-Chave: |
Peptidogalactomannan. |
|
Thesagro: |
Defesa Vegetal; Glicoproteína. |
|
Thesaurus Nal: |
Cladosporium herbarum. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 03116naa a2200289 a 4500
001 2103593
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2018.02.023$2DOI
100 1 $aMATTOS, B. B.
245 $aA peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells.$h[electronic resource]
260 $c2018
520 $aCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively.
650 $aCladosporium herbarum
650 $aDefesa Vegetal
650 $aGlicoproteína
653 $aPeptidogalactomannan
700 1 $aMONTEBIANCO, C.
700 1 $aROMANEL, E.
700 1 $aSILVA, T. da F.
700 1 $aBERNABÉ, R. B.
700 1 $aSIMAS-TOSIN, F.
700 1 $aSOUZA, L. M.
700 1 $aSASSAKI, G. L.
700 1 $aVASLIN, M. F. S.
700 1 $aBARRETO-BERGTER, E.
773 $tPlant Physiology and Biochemistry$gv. 126, p. 206-216, May 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
04/03/2008 |
|
Data da última atualização: |
19/02/2014 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Nacional - A |
|
Autoria: |
ÁVILA, M. R.; BRACCINI, A. de L. e; SCAPIM, C. A.; MANDARINO, J. M. G.; ALBRECHT, L. P.; VIDIGAL FILHO, P. S. |
|
Afiliação: |
MARIZANGELA RIZZATTI ÁVILA, UEM; ALESSANDRO DE LUCCA E BRACCINI, UEM; CARLOS ALBERTO SCAPIM, UEM - bolsista CNPq; JOSÉ MARCOS GONTIJO MANDARINO, CNPSO; LEANDRO PAIOLA ALBRECHT, UEM; PEDRO SOARES VIDIGAL FILHO, UEM - bolsista CNPq. |
|
Título: |
Componentes do rendimento, teores de isoflavonas, proteínas, óleo e qualidade de sementes de soja. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Revista Brasileira de Sementes, Pelotas, v. 29, n. 3, p. 111-127, 2007. |
|
ISSN: |
0101-3122 |
|
DOI: |
http://dx.doi.org/10.1590/S0101-31222007000300014 |
|
Idioma: |
Português |
|
Conteúdo: |
RESUMO: A soja, além de ser utilizada na alimentação animal vem sendo incluída na alimentação humana, pois possui na sua composição química, proteínas, lipídios, algumas vitaminas e compostos polifenólicos, como as isoflavonas. A atividade antioxidante das isoflavonas pode ser considerada um mecanismo de garantia da qualidade das sementes, em virtude da teoria de deterioração, em detrimento da ação de radicais livres na membrana celular. Desta forma, conduziu-se um experimento durante o ano agrícola de 2004/05 em dois locais de semeadura (Maringá e Umuarama), com o objetivo avaliar os componentes de produção, a qualidade fisiológica das sementes de soja produzidas em dois locais de semeadura e determinar os seus teores de óleo, proteínas e de isoflavonas, bem como correlacionar o conteúdo de isoflavonas com os componentes de rendimento, qualidade fisiológica, teores de óleo e proteínas das sementes de soja. As cultivares estudadas foram de ciclo precoce (EMBRAPA 48 e BRS 213) e semiprecoce (BR 36, BRS 133, BRS 184 e BRS 214). Avaliou-se o rendimento e a massa de mil sementes, a qualidade das sementes por meio dos testes de germinação (primeira contagem e contagem final), tetrazólio (vigor e viabilidade) e o teor de óleo, proteínas e isoflavonas. Verificou-se que as cultivares BRS 133 e EMBRAPA 48 apresentaram os maiores conteúdos de isoflavonas, ao passo que, para a cultivar BR 36, foi constatada a menor quantidade, em ambos os locais. Para todas as cultivares avaliadas em Maringá, a germinação das sementes foi superior a 80%. A correlação entre isoflavonas e massa de mil sementes foi negativa para as duas localidades. ABSTRACT: ABSTRACT - Soybean seeds have been used not only for animal, but also for human nutrition because of the presence of proteins; lipids; some vitamins and polyphenolic compounds, such as isoflavones, in their chemical composition. The antioxidant activity of isoflavones may be considered a safe mechanism of seed quality. This is due to the deterioration theory which leads to a cellular detriment because of the action of free radicals on cell membranes. Experiments were carried out during the 2004/05 agricultural year in two different sowing locations (Maringá and Umuarama). The objective of this study was to evaluate the yield, physiological quality of seeds produced in two sowing locations; to determine oil, protein and isoflavone contents; as well as to correlate isoflavone contents to the yield, physiological quality and oil-protein contents of soybean seeds. The seed quality was evaluated by the germination test (first count and final count); the tetrazolium test (vigor and viability) and the concentration of oil, protein and isoflavone tests. Analysis of the isoflavone contents showed that the BRS 133 and EMBRAPA 48 cultivars presented the highest isoflavone contents whereas the BR 36 cultivar showed the lowest isoflavone contents in both places. Germination was above 80% in all cultivars tested in Maringá. The correlation between isoflavones and the weight of one thousand seeds was negative for both locations. MenosRESUMO: A soja, além de ser utilizada na alimentação animal vem sendo incluída na alimentação humana, pois possui na sua composição química, proteínas, lipídios, algumas vitaminas e compostos polifenólicos, como as isoflavonas. A atividade antioxidante das isoflavonas pode ser considerada um mecanismo de garantia da qualidade das sementes, em virtude da teoria de deterioração, em detrimento da ação de radicais livres na membrana celular. Desta forma, conduziu-se um experimento durante o ano agrícola de 2004/05 em dois locais de semeadura (Maringá e Umuarama), com o objetivo avaliar os componentes de produção, a qualidade fisiológica das sementes de soja produzidas em dois locais de semeadura e determinar os seus teores de óleo, proteínas e de isoflavonas, bem como correlacionar o conteúdo de isoflavonas com os componentes de rendimento, qualidade fisiológica, teores de óleo e proteínas das sementes de soja. As cultivares estudadas foram de ciclo precoce (EMBRAPA 48 e BRS 213) e semiprecoce (BR 36, BRS 133, BRS 184 e BRS 214). Avaliou-se o rendimento e a massa de mil sementes, a qualidade das sementes por meio dos testes de germinação (primeira contagem e contagem final), tetrazólio (vigor e viabilidade) e o teor de óleo, proteínas e isoflavonas. Verificou-se que as cultivares BRS 133 e EMBRAPA 48 apresentaram os maiores conteúdos de isoflavonas, ao passo que, para a cultivar BR 36, foi constatada a menor quantidade, em ambos os locais. Para todas as cultivares avaliadas em M... Mostrar Tudo |
|
Palavras-Chave: |
Malonil. |
|
Thesagro: |
Germinação; Glycine Max. |
|
Thesaurus NAL: |
vigor. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97748/1/Componentes-do-rendimento-teores-de-isoflavonas-proteinas-oleo-e-qualidade-de-sementes-de-soja.pdf
|
|
Marc: |
LEADER 03901naa a2200253 a 4500
001 1468672
005 2014-02-19
008 2007 bl uuuu u00u1 u #d
022 $a0101-3122
024 7 $ahttp://dx.doi.org/10.1590/S0101-31222007000300014$2DOI
100 1 $aÁVILA, M. R.
245 $aComponentes do rendimento, teores de isoflavonas, proteínas, óleo e qualidade de sementes de soja.
260 $c2007
520 $aRESUMO: A soja, além de ser utilizada na alimentação animal vem sendo incluída na alimentação humana, pois possui na sua composição química, proteínas, lipídios, algumas vitaminas e compostos polifenólicos, como as isoflavonas. A atividade antioxidante das isoflavonas pode ser considerada um mecanismo de garantia da qualidade das sementes, em virtude da teoria de deterioração, em detrimento da ação de radicais livres na membrana celular. Desta forma, conduziu-se um experimento durante o ano agrícola de 2004/05 em dois locais de semeadura (Maringá e Umuarama), com o objetivo avaliar os componentes de produção, a qualidade fisiológica das sementes de soja produzidas em dois locais de semeadura e determinar os seus teores de óleo, proteínas e de isoflavonas, bem como correlacionar o conteúdo de isoflavonas com os componentes de rendimento, qualidade fisiológica, teores de óleo e proteínas das sementes de soja. As cultivares estudadas foram de ciclo precoce (EMBRAPA 48 e BRS 213) e semiprecoce (BR 36, BRS 133, BRS 184 e BRS 214). Avaliou-se o rendimento e a massa de mil sementes, a qualidade das sementes por meio dos testes de germinação (primeira contagem e contagem final), tetrazólio (vigor e viabilidade) e o teor de óleo, proteínas e isoflavonas. Verificou-se que as cultivares BRS 133 e EMBRAPA 48 apresentaram os maiores conteúdos de isoflavonas, ao passo que, para a cultivar BR 36, foi constatada a menor quantidade, em ambos os locais. Para todas as cultivares avaliadas em Maringá, a germinação das sementes foi superior a 80%. A correlação entre isoflavonas e massa de mil sementes foi negativa para as duas localidades. ABSTRACT: ABSTRACT - Soybean seeds have been used not only for animal, but also for human nutrition because of the presence of proteins; lipids; some vitamins and polyphenolic compounds, such as isoflavones, in their chemical composition. The antioxidant activity of isoflavones may be considered a safe mechanism of seed quality. This is due to the deterioration theory which leads to a cellular detriment because of the action of free radicals on cell membranes. Experiments were carried out during the 2004/05 agricultural year in two different sowing locations (Maringá and Umuarama). The objective of this study was to evaluate the yield, physiological quality of seeds produced in two sowing locations; to determine oil, protein and isoflavone contents; as well as to correlate isoflavone contents to the yield, physiological quality and oil-protein contents of soybean seeds. The seed quality was evaluated by the germination test (first count and final count); the tetrazolium test (vigor and viability) and the concentration of oil, protein and isoflavone tests. Analysis of the isoflavone contents showed that the BRS 133 and EMBRAPA 48 cultivars presented the highest isoflavone contents whereas the BR 36 cultivar showed the lowest isoflavone contents in both places. Germination was above 80% in all cultivars tested in Maringá. The correlation between isoflavones and the weight of one thousand seeds was negative for both locations.
650 $avigor
650 $aGerminação
650 $aGlycine Max
653 $aMalonil
700 1 $aBRACCINI, A. de L. e
700 1 $aSCAPIM, C. A.
700 1 $aMANDARINO, J. M. G.
700 1 $aALBRECHT, L. P.
700 1 $aVIDIGAL FILHO, P. S.
773 $tRevista Brasileira de Sementes, Pelotas$gv. 29, n. 3, p. 111-127, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
