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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
04/01/2024 |
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Data da última atualização: |
04/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
F. C. SANTOS, UDI PESQUISA & DESENVOLVIMENTO; A. C. S. CLEMENTE, UNIVERSIDADE FEDERAL DE LAVRAS; F. CAIXETA, GENERAL MILLS BRASIL ALIMENTOS LTDA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa. |
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Título: |
Endo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4 p. 11719-11728, 2015. |
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DOI: |
http://dx.doi.org/10.4238/2015.October.2.5 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed matur... Mostrar Tudo |
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Thesaurus Nal: |
Coffea arabica var. arabica; Developmental stages; Organogenesis; Seed maturation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/260321/1/Endo-mannanase.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2160448
005 2024-01-04
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2015.October.2.5$2DOI
100 1 $aSANTOS, F. C.
245 $aEndo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation.
650 $aCoffea arabica var. arabica
650 $aDevelopmental stages
650 $aOrganogenesis
650 $aSeed maturation
700 1 $aCLEMENTE, A. C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4 p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
02/03/2000 |
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Data da última atualização: |
25/02/2019 |
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Autoria: |
ARAUJO, J. M. de A.; NICOLINO, A. P. N.; BLATT, C. |
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Afiliação: |
JÚLIO MARIA DE ANDRADE ARAÚJO, Universidade Federal de Viçosa- UFV/Departamento de Tecnologia de Alimentos; ANA PAULA NOGUEIRA NICOLINO, Universidade Federal de Viçosa- UFV; CELSO BLATT, HP Brasil. |
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Título: |
Utilização do dióxido de carbono supercritico na concentração de tocoferois do destilado desodorizado do óleo de soja. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 1, p. 201-205, jan. 2000. |
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Idioma: |
Português |
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Notas: |
Título em inglês: Utilization of supercritical carbon dioxide for concentration of tocopher ols |
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Conteúdo: |
O objetivo deste trabalho foi examinar a possibilidade do uso do dioxido de carbono supercritico na extracao e concentracao de tocoferois a partir do destilado desodorizado do oleo de soja. Trata-se da combinacao sequencial de duas extracoes: pre-extracao a temperatura de 80.C e pressao de 76 bars para remocao de substancias interferentes e extracao dos tocoferois a 50.C e 197 bars. O coletor utilizado foi o octadesil-silica, lavado com acetonitrila na pre-extracao e hexano na extracao, e a analise dos extratos obtidos foi feita por cromatografia de fase gasosa em coluna capilar. O resultado mostrou que o desodorizado do oleo de soja, contendo inicialmente 9,2% de tocoferois totais, pode ser concentrado para 40,6%. Sob as condicoes otimizadas, a extracao pode ser realizada em 37 minutos. |
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Palavras-Chave: |
Supercritical extraction. |
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Thesagro: |
Cromatografia; Extração Supercrítica; Soja. |
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Thesaurus NAL: |
gas chromatography; soybeans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/16783/1/pab97_163.pdf
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Marc: |
LEADER 01605naa a2200229 a 4500
001 1101273
005 2019-02-25
008 2000 bl uuuu u00u1 u #d
100 1 $aARAUJO, J. M. de A.
245 $aUtilização do dióxido de carbono supercritico na concentração de tocoferois do destilado desodorizado do óleo de soja.
260 $c2000
500 $aTítulo em inglês: Utilization of supercritical carbon dioxide for concentration of tocopher ols
520 $aO objetivo deste trabalho foi examinar a possibilidade do uso do dioxido de carbono supercritico na extracao e concentracao de tocoferois a partir do destilado desodorizado do oleo de soja. Trata-se da combinacao sequencial de duas extracoes: pre-extracao a temperatura de 80.C e pressao de 76 bars para remocao de substancias interferentes e extracao dos tocoferois a 50.C e 197 bars. O coletor utilizado foi o octadesil-silica, lavado com acetonitrila na pre-extracao e hexano na extracao, e a analise dos extratos obtidos foi feita por cromatografia de fase gasosa em coluna capilar. O resultado mostrou que o desodorizado do oleo de soja, contendo inicialmente 9,2% de tocoferois totais, pode ser concentrado para 40,6%. Sob as condicoes otimizadas, a extracao pode ser realizada em 37 minutos.
650 $agas chromatography
650 $asoybeans
650 $aCromatografia
650 $aExtração Supercrítica
650 $aSoja
653 $aSupercritical extraction
700 1 $aNICOLINO, A. P. N.
700 1 $aBLATT, C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 35, n. 1, p. 201-205, jan. 2000.
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Embrapa Unidades Centrais (AI-SEDE) |
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