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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
27/10/2010 |
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Data da última atualização: |
04/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, G. T. C. P.; CARNEIRO, N. P.; KARTHIKEYAN, A. S.; RAGHOTHAMA, K. G.; SCHAFFERT, R. E.; BRANDAO, R. L.; PAIVA, L. V.; SOUZA, I. R. P. de; ALVES, V. M.; IMOLESI, A.; CARVALHO, C. H. S.; CARNEIRO, A. A. |
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Afiliação: |
Gracielle T. C. P. Coelho, UFLA; NEWTON PORTILHO CARNEIRO, CNPMS; ATHIKKATTUVALASU S. KARTHIKEYAN, Purdue University; KASCHANDRA G. RAGHOTHAMA, Purdue University; ROBERT EUGENE SCHAFFERT, CNPMS; ROSANGELA L. BRANDÃO, UFLA; LUCIANO V. PAIVA, UFLA; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; ANDERSON IMOLESI; CARLOS H. S. CARVALHO; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
A phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 28, p. 717-723, 2010. |
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DOI: |
10.1007/s1 I 105-010-0199-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Phosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions. |
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Thesagro: |
Fósforo; Genética vegetal. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01970naa a2200289 a 4500
001 1865352
005 2018-06-04
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s1 I 105-010-0199-8$2DOI
100 1 $aCOELHO, G. T. C. P.
245 $aA phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation.$h[electronic resource]
260 $c2010
520 $aPhosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.
650 $aFósforo
650 $aGenética vegetal
700 1 $aCARNEIRO, N. P.
700 1 $aKARTHIKEYAN, A. S.
700 1 $aRAGHOTHAMA, K. G.
700 1 $aSCHAFFERT, R. E.
700 1 $aBRANDAO, R. L.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA, I. R. P. de
700 1 $aALVES, V. M.
700 1 $aIMOLESI, A.
700 1 $aCARVALHO, C. H. S.
700 1 $aCARNEIRO, A. A.
773 $tPlant Molecular Biology Reporter, Athens$gv. 28, p. 717-723, 2010.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
27/09/2012 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAÚJO, E. R.; COSTA, J. R.; FERREIRA, M. A. S. V.; QUEZADO-DUVAL, A. M. |
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Afiliação: |
ALICE MARIA QUEZADO DUVAL, CNPH. |
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Título: |
Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, Oxford, Sept. 2012. |
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ISSN: |
1364-5072 |
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DOI: |
10.1111/j.1365-2672.05431.x |
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Idioma: |
Inglês |
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Conteúdo: |
To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification. |
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Palavras-Chave: |
Detecção; PCR. |
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Thesagro: |
Bactéria; Doença; Identificação. |
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Thesaurus NAL: |
Xanthomonas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177608/1/Ara-jo-et-al-2012-Journal-of-Applied-Microbiology.pdf
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Marc: |
LEADER 01335naa a2200253 a 4500
001 1934722
005 2018-05-24
008 2012 bl uuuu u00u1 u #d
022 $a1364-5072
024 7 $a10.1111/j.1365-2672.05431.x$2DOI
100 1 $aARAÚJO, E. R.
245 $aSimultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.$h[electronic resource]
260 $c2012
520 $aTo establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
650 $aXanthomonas
650 $aBactéria
650 $aDoença
650 $aIdentificação
653 $aDetecção
653 $aPCR
700 1 $aCOSTA, J. R.
700 1 $aFERREIRA, M. A. S. V.
700 1 $aQUEZADO-DUVAL, A. M.
773 $tJournal of Applied Microbiology, Oxford, Sept. 2012.
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