01970naa a2200289 a 450000100080000000500110000800800410001902400370006010000240009724501910012126000090031252010320032165000130135365000220136670000200138870000230140870000220143170000210145370000190147470000170149370000230151070000170153370000160155070000230156670000200158977300710160918653522018-06-04       2010    bl uuuu     u00u1 u    #d7 a10.1007/s1 I 105-010-0199-82DOI1 aCOELHO, G. T. C. P.  aA phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation.h[electronic resource]  c2010  aPhosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.  aFósforo  aGenética vegetal1 aCARNEIRO, N. P.1 aKARTHIKEYAN, A. S.1 aRAGHOTHAMA, K. G.1 aSCHAFFERT, R. E.1 aBRANDAO, R. L.1 aPAIVA, L. V.1 aSOUZA, I. R. P. de1 aALVES, V. M.1 aIMOLESI, A.1 aCARVALHO, C. H. S.1 aCARNEIRO, A. A.  tPlant Molecular Biology Reporter, Athensgv. 28, p. 717-723, 2010.