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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
29/12/2022 |
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Data da última atualização: |
15/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefnery, v. 14, n. 8, 2022. |
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Páginas: |
9121-9136 |
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DOI: |
https://doi.org/10.1007/s13399-022-02977-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. MenosThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stabili... Mostrar Tudo |
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Palavras-Chave: |
Endoglucanase; Packed-bed bioreactor; Solid-state cultivation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02537naa a2200229 a 4500
001 2150493
005 2024-05-15
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13399-022-02977-1$2DOI
100 1 $aKATAYAMA, E.
245 $aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.$h[electronic resource]
260 $c2022
300 $a9121-9136
520 $aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.
653 $aEndoglucanase
653 $aPacked-bed bioreactor
653 $aSolid-state cultivation
700 1 $aRODRIGUES, N. A.
700 1 $aBILATTO, S.
700 1 $aCASCIATORI, F. P.
700 1 $aFARINAS, C. S.
773 $tBiomass Conversion and Biorefnery$gv. 14, n. 8, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/04/1992 |
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Data da última atualização: |
27/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
ALMEIDA, A. M. R. |
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Afiliação: |
EMBRAPA-CNPSo. Londrina, PR. |
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Título: |
Acao de diferentes temperaturas e regimes de luz sobre a germinacao dos esporos de Septoria glycines Hemmi. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, v. 3, n. 2, p. 211-214, 1978. |
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Idioma: |
Português |
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Conteúdo: |
Em trabalhos de pesquisa de fonte de resistencia a Septoria glycines observou-se a necessidade de utilizar extenso periodo de incubacao para plantas de soja inoculadas, bem como utilizar alta concentracao de esporos a fim de se obter infeccao ideal para avaliacoes. Procurou-se avaliar inicialmente, o efeito de temperatura e luminosidade sobre a germinacao dos esporos de S. glycines. Luz nao afetou a germinacao. Incubacao por 24 horas a temperatura de 25C induziu maior germinacao (43%). Com 48 hs. de incubacao as temperaturas de 22, 25 e 28oC apresentaram resultados semelhantes, apresentando cerca de 83% de germinacao. As temperaturas de 20 e 30oC nao induziram aumento de germinacao. |
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Palavras-Chave: |
Disease; Fitopatologia; Phytopathology; Soybean. |
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Thesagro: |
Doença; Septoria Glycines; Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01317naa a2200217 a 4500
001 1457330
005 2024-02-27
008 1978 bl uuuu u00u1 u #d
100 1 $aALMEIDA, A. M. R.
245 $aAcao de diferentes temperaturas e regimes de luz sobre a germinacao dos esporos de Septoria glycines Hemmi.$h[electronic resource]
260 $c1978
520 $aEm trabalhos de pesquisa de fonte de resistencia a Septoria glycines observou-se a necessidade de utilizar extenso periodo de incubacao para plantas de soja inoculadas, bem como utilizar alta concentracao de esporos a fim de se obter infeccao ideal para avaliacoes. Procurou-se avaliar inicialmente, o efeito de temperatura e luminosidade sobre a germinacao dos esporos de S. glycines. Luz nao afetou a germinacao. Incubacao por 24 horas a temperatura de 25C induziu maior germinacao (43%). Com 48 hs. de incubacao as temperaturas de 22, 25 e 28oC apresentaram resultados semelhantes, apresentando cerca de 83% de germinacao. As temperaturas de 20 e 30oC nao induziram aumento de germinacao.
650 $aSoybeans
650 $aDoença
650 $aSeptoria Glycines
650 $aSoja
653 $aDisease
653 $aFitopatologia
653 $aPhytopathology
653 $aSoybean
773 $tFitopatologia Brasileira$gv. 3, n. 2, p. 211-214, 1978.
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Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Pantanal. |   |
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