02537naa a2200229 a 450000100080000000500110000800800410001902400520006010000170011224501820012926000090031130000140032052017650033465300180209965300260211765300280214370000210217170000160219270000220220870000190223077300580224921504932024-05-15       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s13399-022-02977-12DOI1 aKATAYAMA, E.  aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.h[electronic resource]  c2022  a9121-9136  aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.  aEndoglucanase  aPacked-bed bioreactor  aSolid-state cultivation1 aRODRIGUES, N. A.1 aBILATTO, S.1 aCASCIATORI, F. P.1 aFARINAS, C. S.  tBiomass Conversion and Biorefnerygv. 14, n. 8, 2022.