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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/04/2015 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
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Título: |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 6, e98758, 2014. |
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DOI: |
https://doi.org/10.1371/journal.pone.0098758 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Palavras-Chave: |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122336/1/Cnpgl-2014-PlosOne-Evaluation.pdf
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Marc: |
LEADER 02105naa a2200301 a 4500
001 2013504
005 2024-01-30
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0098758$2DOI
100 1 $aDORNELES, E. M. S.
245 $aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
653 $aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
700 1 $aSANTANA, J. A.
700 1 $aRIBEIRO, D.
700 1 $aDORELLA, F. A.
700 1 $aGUIMARAES, A. S.
700 1 $aMOAWAD, M. S.
700 1 $aSELIM, S. A.
700 1 $aGARALDI, A. L. M.
700 1 $aMIYOSHI, A.
700 1 $aRIBEIRO, M. G.
700 1 $aGOUVEIA, A. M. G.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tPlos One$gv. 9, n. 6, e98758, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
01/02/2012 |
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Data da última atualização: |
01/02/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CESTONARO, T.; LOURENÇO, K. S.; CORREA, J. C.; ABREU, V. M. N. |
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Afiliação: |
TAIANA CESTONARO, UNC; UFSC; JULIANO CORULLI CORREA, CNPSA; VALERIA MARIA NASCIMENTO ABREU, CNPSA. |
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Título: |
Diferentes tipos e doses de cama de frango na cultura do feijão. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 2., 2011, Foz do Iguaçu. Anais... Concórdia: Embrapa Suínos e Aves, 2011. v. 2. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
Projeto; 01.09.01.001. |
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Palavras-Chave: |
Cama; Nutrientes. |
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Thesagro: |
Avicultura; Feijão; Fósforo; Nitrogênio; Potássio. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53315/1/diferentes-tipos-e-doses.pdf
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Marc: |
LEADER 00779nam a2200229 a 4500
001 1914132
005 2012-02-01
008 2011 bl uuuu u00u1 u #d
100 1 $aCESTONARO, T.
245 $aDiferentes tipos e doses de cama de frango na cultura do feijão.$h[electronic resource]
260 $aIn: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 2., 2011, Foz do Iguaçu. Anais... Concórdia: Embrapa Suínos e Aves, 2011. v. 2. 1 CD-ROM.$c2011
500 $aProjeto; 01.09.01.001.
650 $aAvicultura
650 $aFeijão
650 $aFósforo
650 $aNitrogênio
650 $aPotássio
653 $aCama
653 $aNutrientes
700 1 $aLOURENÇO, K. S.
700 1 $aCORREA, J. C.
700 1 $aABREU, V. M. N.
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Embrapa Suínos e Aves (CNPSA) |
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