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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
Data corrente: |
21/02/2022 |
Data da última atualização: |
05/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C. |
Afiliação: |
EDUARDO ANDRÉ ROESLER, UPF; NATALIA BALBINOTT, UFRGS; RENATA GABRIELA SCHROEDER, UPF; ELENE YAMAZAKI LAU, CNPT; MARIA IMACULADA PONTES M LIMA, CNPT; SANDRA PATUSSI BRAMMER, CNPT; FRANCISCO JOSE LIMA ARAGAO, Cenargen; JOSE MAURICIO CUNHA FERNANDES, CNPT; CAROLINA CARDOSO DEUNER, UPF. |
Título: |
An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Tropical Plant Pathology, 16 Feb. 2022 |
DOI: |
https://doi.org/10.1007/s40858-022-00497-x |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis. |
Palavras-Chave: |
Inoculation assay; Protocol. |
Thesagro: |
Doença Fúngica; Ferrugem; Fusarium Graminearum; Trigo. |
Thesaurus Nal: |
Arabidopsis thaliana; Fusarium head blight. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02292naa a2200325 a 4500 001 2140281 005 2022-09-05 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s40858-022-00497-x$2DOI 100 1 $aROESLER, E. A. 245 $aAn efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.$h[electronic resource] 260 $c2022 520 $aAbstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis. 650 $aArabidopsis thaliana 650 $aFusarium head blight 650 $aDoença Fúngica 650 $aFerrugem 650 $aFusarium Graminearum 650 $aTrigo 653 $aInoculation assay 653 $aProtocol 700 1 $aBALBINOTT, N. 700 1 $aSCHROEDER, R. G. 700 1 $aLAU, E. Y. 700 1 $aLIMA, M. I. P. M. 700 1 $aBRAMMER, S. P. 700 1 $aARAGÃO, F. J. L. 700 1 $aFERNANDES, J. M. C. 700 1 $aDEUNER, C. C. 773 $tTropical Plant Pathology, 16 Feb. 2022
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Embrapa Trigo (CNPT) |
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Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
10/03/2017 |
Data da última atualização: |
13/03/2017 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
BREDEMEIER, C.; VIAN, A. L.; PIRES, J. L. F. |
Afiliação: |
CHRISTIAN BREDEMEIER, UFRGS; ANDRÉ LUIS VIAN, UFRGS; JOAO LEONARDO FERNANDES PIRES, CNPT. |
Título: |
Aplicação de nitrogênio em tempo real: modelos e aplicações. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: SANTI, A. L.; GIOTTO, E.; SEBEM, E.; AMADO, T. J. C. (Org.). Agricultura de precisão no Rio Grande do Sul. Santa Maria: CESPOL, 2016. Cap. 7, p. 137-158. |
Idioma: |
Português |
Palavras-Chave: |
Adubação nitogenada; Sensor de vegetação; Taxa variável. |
Thesagro: |
Agricultura de Precisão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157541/1/ID43966-2016LV-AgriculturaPrecisaoCap7.pdf
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Marc: |
LEADER 00662naa a2200181 a 4500 001 2066816 005 2017-03-13 008 2016 bl uuuu u00u1 u #d 100 1 $aBREDEMEIER, C. 245 $aAplicação de nitrogênio em tempo real$bmodelos e aplicações.$h[electronic resource] 260 $c2016 650 $aAgricultura de Precisão 653 $aAdubação nitogenada 653 $aSensor de vegetação 653 $aTaxa variável 700 1 $aVIAN, A. L. 700 1 $aPIRES, J. L. F. 773 $tIn: SANTI, A. L.; GIOTTO, E.; SEBEM, E.; AMADO, T. J. C. (Org.). Agricultura de precisão no Rio Grande do Sul. Santa Maria: CESPOL, 2016. Cap. 7, p. 137-158.
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