02292naa a2200325 a 450000100080000000500110000800800410001902400520006010000190011224501240013126000090025552013460026465000250161065000250163565000210166065000130168165000250169465000100171965300220172965300130175170000180176470000210178270000150180370000220181870000190184070000220185970000240188170000180190577300430192321402812022-09-05       2022    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s40858-022-00497-x2DOI1 aROESLER, E. A.  aAn efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.h[electronic resource]  c2022  aAbstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis.  aArabidopsis thaliana  aFusarium head blight  aDoença Fúngica  aFerrugem  aFusarium Graminearum  aTrigo  aInoculation assay  aProtocol1 aBALBINOTT, N.1 aSCHROEDER, R. G.1 aLAU, E. Y.1 aLIMA, M. I. P. M.1 aBRAMMER, S. P.1 aARAGÃO, F. J. L.1 aFERNANDES, J. M. C.1 aDEUNER, C. C.  tTropical Plant Pathology, 16 Feb. 2022