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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
25/06/2003 |
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Data da última atualização: |
28/09/2010 |
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Autoria: |
SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A. |
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Afiliação: |
JOSE ROBSON BEZERRA SERENO, CPAP. |
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Título: |
Bases de la caracterización del cerdo ibérico por la aptitud sexual del macho como clave para su conservación. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.11-19, ene., 2003. |
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Idioma: |
Espanhol |
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Conteúdo: |
En los últimos años las distintas variedades que configuran la raza porcina de cerdo Ibérico han sido caracterizadas desde el punto de vista de la conservación en los ámbitos morfológico, reproductivo y genético quedando pendiente la caracterización de la aptitud sexual del macho, siendo ésta el objetivo central de esta monografia. En el presente capítulo hacemos referencia al interés del conocimiento del comportamiento sexual del cerdo Ibérico abordando las bases del entrenamiento de los sementales frente al maniquí, el proceso de extracción de semen, la frecuencia de extracción seminal y las propias particularidades de esta área del comportamiento. Asimismo también se pone de manifiesto la importancia y aplicación de este comportamiento relacionado con otros factores importantes dentro de los programas de reproducción asistida como son la detección del celo, el momento de la ovolación y el momento óptimo de la inseminación artificial. |
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Palavras-Chave: |
Conservation; Iberian pib; Porco ibérico. |
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Thesagro: |
Comportamento Sexual; Conservação; Inseminação Artificial; Reprodução. |
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Thesaurus Nal: |
reproduction. |
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Categoria do assunto: |
-- |
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URL: |
https://www.aulaveterinaria.com
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Marc: |
LEADER 01780naa a2200265 a 4500
001 1790661
005 2010-09-28
008 2003 bl --- 0-- u #d
100 1 $aSERENO, J. R. B.
245 $aBases de la caracterización del cerdo ibérico por la aptitud sexual del macho como clave para su conservación.
260 $c2003
520 $aEn los últimos años las distintas variedades que configuran la raza porcina de cerdo Ibérico han sido caracterizadas desde el punto de vista de la conservación en los ámbitos morfológico, reproductivo y genético quedando pendiente la caracterización de la aptitud sexual del macho, siendo ésta el objetivo central de esta monografia. En el presente capítulo hacemos referencia al interés del conocimiento del comportamiento sexual del cerdo Ibérico abordando las bases del entrenamiento de los sementales frente al maniquí, el proceso de extracción de semen, la frecuencia de extracción seminal y las propias particularidades de esta área del comportamiento. Asimismo también se pone de manifiesto la importancia y aplicación de este comportamiento relacionado con otros factores importantes dentro de los programas de reproducción asistida como son la detección del celo, el momento de la ovolación y el momento óptimo de la inseminación artificial.
650 $areproduction
650 $aComportamento Sexual
650 $aConservação
650 $aInseminação Artificial
650 $aReprodução
653 $aConservation
653 $aIberian pib
653 $aPorco ibérico
700 1 $aDELGADO, J. V.
700 1 $aSERENO, F. T. P. S.
700 1 $aBARBA, C.
700 1 $aCABELLO, A.
773 $tIn: Porci. Aula Veterinária - Monografias de actualidad, Madrid$gn.73, p.11-19, ene., 2003.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Biblioteca |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A novel soybean hairy root system for gene functional validation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
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DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
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Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
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Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
LEADER 02598naa a2200337 a 4500
001 2154066
005 2024-04-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI
100 1 $aPEREIRA, B. M.
245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource]
260 $c2023
500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
650 $aSoybeans
650 $aGlycine Max
650 $aMelhoramento Genético Vegetal
650 $aMeloidogyne spp
650 $aSoja
653 $aAgrobacterium rhizogenes
653 $aTransgenic soybean
700 1 $aARRAES, F.
700 1 $aMARTINS, A. C. Q.
700 1 $aALVES, N. S. F.
700 1 $aMELO, B. P.
700 1 $aMORGANTE, C. V.
700 1 $aSARAIVA, M. A. P.
700 1 $aSA, M. F. G. de
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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