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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Pecuária Sudeste. |
Data corrente: |
02/10/2018 |
Data da última atualização: |
02/10/2018 |
Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
Autoria: |
SANTOS, C. E. S.; NICODEMO, M. L. F.; BORGES, W. L. B.; SANTOS JUNIOR, H. A. dos. |
Afiliação: |
CARLOS EDUARDO SILVA SANTOS, CPAC; MARIA LUIZA FRANCESCHI NICODEMO, CPPSE; WANDER LUIS BARBOSA BORGES, Instituto Agronônomico, Votuporanga, SP; HAMILTON ANTÔNIO DOS SANTOS JÚNIOR, Coordenadoria de Assistência Técnica Integral, Ibirá, SP. |
Título: |
Sistemas silvipastoris: relação de parceria na construção do conhecimento local. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2018. |
Páginas: |
37 p. |
Descrição Física: |
il.: color. |
Série: |
(Sistematização de experiências : métodos de transferência de tecnologia, intercâmbio e construção do conhecimento, v. 14) |
ISBN: |
978-85-7035-832-5 |
Idioma: |
Português |
Palavras-Chave: |
ILPF. |
Thesagro: |
Agrossilvicultura; Controle Integrado; Produção Integrada; Transferência de Tecnologia. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183780/1/COLECAO-SISTEMATIZACAO-EXPERIENCIAS-SISTEMAS-SILVIPASTORIS-vol-14-apagar.pdf
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Marc: |
LEADER 00821nam a2200229 a 4500 001 2096733 005 2018-10-02 008 2018 bl uuuu 00u1 u #d 020 $a978-85-7035-832-5 100 1 $aSANTOS, C. E. S. 245 $aSistemas silvipastoris$brelação de parceria na construção do conhecimento local.$h[electronic resource] 260 $aBrasília, DF: Embrapa$c2018 300 $a37 p.$cil.: color. 490 $a(Sistematização de experiências : métodos de transferência de tecnologia, intercâmbio e construção do conhecimento, v. 14) 650 $aAgrossilvicultura 650 $aControle Integrado 650 $aProdução Integrada 650 $aTransferência de Tecnologia 653 $aILPF 700 1 $aNICODEMO, M. L. F. 700 1 $aBORGES, W. L. B. 700 1 $aSANTOS JUNIOR, H. A. dos
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Embrapa Cerrados (CPAC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
24/04/2021 |
Data da última atualização: |
12/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
ISSN: |
1874-3919 |
DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
Idioma: |
Inglês Português |
Notas: |
Na publicação: Angela Mehta. |
Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
Thesaurus NAL: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 03484naa a2200469 a 4500 001 2131497 005 2021-07-12 008 2021 bl uuuu u00u1 u #d 022 $a1874-3919 024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI 100 1 $aTÁVORA, F. T. P. K. 245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource] 260 $c2021 500 $aNa publicação: Angela Mehta. 520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. 650 $ablast disease 650 $adisease resistance 650 $afoliar diseases 650 $aMagnaporthe oryzae 650 $aplant diseases and disorders 650 $aproteomics 650 $arice 650 $aArroz 650 $aBrusone 650 $aDoença de Planta 650 $aOryza Sativa 653 $aGene ontology 653 $aPlant immunity 653 $aPTO-based TIGS 653 $aRT-qPCR 653 $aS-gene 653 $aTransient-Induced gene silencing 700 1 $aBEVITORI, R. 700 1 $aMELLO, R. N. de 700 1 $aCINTRA, M. M. D. F. 700 1 $aOLIVEIRA NETO, O. B. 700 1 $aFONTES, W. 700 1 $aCASTRO, M. S. 700 1 $aSOUSA, M. V. 700 1 $aFRANCO, O. L. 700 1 $aREIS, A. M. dos 773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
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