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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/08/1995 |
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Data da última atualização: |
17/04/2023 |
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Autoria: |
SHALITIN, D.; MAWASSI, M.; GAFNY, R.; LEITNER, O.; CABILLY, S.; ESHHAR, Z.; BAR-JOSEPH, M. |
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Afiliação: |
Department of Biological Sciences, Department of Membrane and Biophysics and Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel. |
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Título: |
Serological characterisation of citrus tristeza virus isolates from Israel. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Annals of Applied Biology, v.125, n.1, p.105-113, 1994 |
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ISSN: |
0003-4746 |
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Idioma: |
Inglês |
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Conteúdo: |
Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reaction in ELISA with polyclonal antibodies and with one MAb (#25/2). Two MAbs; #13/21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG-VT group (Mawassi, Gafny & Bar-Joseph, 1993) reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG-MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV-CPG. |
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Palavras-Chave: |
Aphid transmissibility; Coat protein gene; Pathogenic isolates; Tristeza-epidemiology. |
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Thesaurus Nal: |
monoclonal antibodies. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1633970
005 2023-04-17
008 1994 bl uuuu u00u1 u #d
022 $a0003-4746
100 1 $aSHALITIN, D.
245 $aSerological characterisation of citrus tristeza virus isolates from Israel.$h[electronic resource]
260 $c1994
520 $aSeven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reaction in ELISA with polyclonal antibodies and with one MAb (#25/2). Two MAbs; #13/21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG-VT group (Mawassi, Gafny & Bar-Joseph, 1993) reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG-MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV-CPG.
650 $amonoclonal antibodies
653 $aAphid transmissibility
653 $aCoat protein gene
653 $aPathogenic isolates
653 $aTristeza-epidemiology
700 1 $aMAWASSI, M.
700 1 $aGAFNY, R.
700 1 $aLEITNER, O.
700 1 $aCABILLY, S.
700 1 $aESHHAR, Z.
700 1 $aBAR-JOSEPH, M.
773 $tAnnals of Applied Biology$gv.125, n.1, p.105-113, 1994
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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