01934naa a2200265 a 450000100080000000500110000800800410001902200140006010000170007424501030009126000090019452011850020365000260138865300270141465300220144165300240146365300260148770000160151370000140152970000160154370000160155970000150157570000190159077300590160916339702023-04-17       1994    bl uuuu     u00u1 u    #d  a0003-47461 aSHALITIN, D.  aSerological characterisation of citrus tristeza virus isolates from Israel.h[electronic resource]  c1994  aSeven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reaction in ELISA with polyclonal antibodies and with one MAb (#25/2). Two MAbs; #13/21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG-VT group (Mawassi, Gafny & Bar-Joseph, 1993) reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG-MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV-CPG.  amonoclonal antibodies  aAphid transmissibility  aCoat protein gene  aPathogenic isolates  aTristeza-epidemiology1 aMAWASSI, M.1 aGAFNY, R.1 aLEITNER, O.1 aCABILLY, S.1 aESHHAR, Z.1 aBAR-JOSEPH, M.  tAnnals of Applied Biologygv.125, n.1, p.105-113, 1994