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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/05/2024 |
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Data da última atualização: |
13/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, I. A.; HORTA, R. P.; MATOS, M.; HELM, C. V.; MAGALHAES, W. L. E.; LIMA, E. A. de; SILVA, B. J. G. da; MUNIZ, G. I. B. de; CADEMARTORI, P. H. G. de. |
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Afiliação: |
IVANA AMORIM DIAS, UNIVERSIDADE FEDERAL DO PARANÁ; RAYTA PAIM HORTA, UNIVERSIDADE FEDERAL DO PARANÁ; MAILSON MATOS, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; EDSON ALVES DE LIMA, CNPF; BRUNO JOSÉ GONÇALVES DA SILVA, UNIVERSIDADE FEDERAL DO PARANÁ; GRACIELA INES BOLZON DE MUNIZ, UNIVERSIDADE FEDERAL DO PARANÁ; PEDRO HENRIQUE GONZALEZ DE CADEMARTORI, UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Exploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefinery, p. 1-12, 2023. |
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DOI: |
http://dx.doi.org/10.1007/s13399-023-04561-7 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
Improving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities. MenosImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal act... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial potential; Aqueous fraction; Levoglucosan; Storage stability. |
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Thesaurus Nal: |
Antioxidant activity; Phenolic compounds. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 03046naa a2200313 a 4500
001 2164243
005 2024-05-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1007/s13399-023-04561-7$2DOI
100 1 $aDIAS, I. A.
245 $aExploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil.$h[electronic resource]
260 $c2023
500 $aOnline first.
520 $aImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities.
650 $aAntioxidant activity
650 $aPhenolic compounds
653 $aAntimicrobial potential
653 $aAqueous fraction
653 $aLevoglucosan
653 $aStorage stability
700 1 $aHORTA, R. P.
700 1 $aMATOS, M.
700 1 $aHELM, C. V.
700 1 $aMAGALHAES, W. L. E.
700 1 $aLIMA, E. A. de
700 1 $aSILVA, B. J. G. da
700 1 $aMUNIZ, G. I. B. de
700 1 $aCADEMARTORI, P. H. G. de
773 $tBiomass Conversion and Biorefinery, p. 1-12, 2023.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Semiárido. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, L. M. B.; ALONSO, J. D.; KIILL, C. P.; FERREIRA, N. N.; BUZZÁ, H. H.; GODOI, D. R. M. de; BRITTO, D. de; ASSIS, O. B. G. de; SERAPHIM, T. V.; BORGES, J. C.; GREMIÃO, M. P. D. |
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Afiliação: |
DOUGLAS DE BRITTO, CPATSA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA. |
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Título: |
Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
European Polymer Journal, v. 103, p. 238-250, jun. 2018. |
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Páginas: |
238-250 |
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DOI: |
10.1016/j.eurpolymj.2018.04.013 |
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Idioma: |
Inglês |
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Conteúdo: |
Monoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers. MenosMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited di@... Mostrar Tudo |
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Palavras-Chave: |
Bevacizumab; Drug delivery platform; Interações supramoleculares; Mucoadesão; Mucoadhesion; Nanoparticula; Nanopartículas poliméricas; Penetração do muco; Plataforma de entrega de medicamentos; Polymeric nanoparticles; Supramolecular interactions. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03262naa a2200397 a 4500
001 2096760
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.eurpolymj.2018.04.013$2DOI
100 1 $aFERREIRA, L. M. B.
245 $aExploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery.$h[electronic resource]
260 $c2018
300 $a238-250
520 $aMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers.
653 $aBevacizumab
653 $aDrug delivery platform
653 $aInterações supramoleculares
653 $aMucoadesão
653 $aMucoadhesion
653 $aNanoparticula
653 $aNanopartículas poliméricas
653 $aPenetração do muco
653 $aPlataforma de entrega de medicamentos
653 $aPolymeric nanoparticles
653 $aSupramolecular interactions
700 1 $aALONSO, J. D.
700 1 $aKIILL, C. P.
700 1 $aFERREIRA, N. N.
700 1 $aBUZZÁ, H. H.
700 1 $aGODOI, D. R. M. de
700 1 $aBRITTO, D. de
700 1 $aASSIS, O. B. G. de
700 1 $aSERAPHIM, T. V.
700 1 $aBORGES, J. C.
700 1 $aGREMIÃO, M. P. D.
773 $tEuropean Polymer Journal$gv. 103, p. 238-250, jun. 2018.
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