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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
20/06/2023 |
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Autoria: |
GOEL, M. C.; SINGH, I. P. |
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Título: |
Purification and characterization of Corynebacterium ovis exotoxin. |
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Ano de publicação: |
1972 |
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Fonte/Imprenta: |
Journal of Comparative Pathology, v. 82, n. 3, p. 345-353, Jul. 1972. |
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DOI: |
https://doi.org/10.1016/0021-9975(72)90016-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A scheme for the purification of Corynebacterium ovis toxin by fractional precipitation with ammonium sulphate has been described. Based on four experiments a mean yield of 219·1 per cent. of the original activity was obtained. The mean concentration effected was 96-fold. Expressing the activity of the toxin per mg. of non-dialysable nitrogen, the mean purification value achieved was 361·3 per cent. The dermo-necrotic and staphylococcal beta-haemolysin inhibition activities of C. ovis toxin behaved identically to precipitation by ammonium sulphate, inactivation with formaldehyde and heat, neutralization by antitoxin and fractionation by Sephadex G-100. These two activities are considered to be the manifestation of the same protein moiety. The toxin appears to be composed of two components, one a stable high molecular weight and the other an unstable low molecular weight protein. |
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Palavras-Chave: |
Ammonium sulphate; Caracterização. |
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Thesagro: |
Corynebacterium Ovis; Doença Animal; Linfadenite Caseosa. |
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Thesaurus Nal: |
Animal diseases; Application methods; Caseous lymphadenitis; Exotoxins. |
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Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01659naa a2200253 a 4500
001 1516532
005 2023-06-20
008 1972 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0021-9975(72)90016-3$2DOI
100 1 $aGOEL, M. C.
245 $aPurification and characterization of Corynebacterium ovis exotoxin.
260 $c1972
520 $aAbstract: A scheme for the purification of Corynebacterium ovis toxin by fractional precipitation with ammonium sulphate has been described. Based on four experiments a mean yield of 219·1 per cent. of the original activity was obtained. The mean concentration effected was 96-fold. Expressing the activity of the toxin per mg. of non-dialysable nitrogen, the mean purification value achieved was 361·3 per cent. The dermo-necrotic and staphylococcal beta-haemolysin inhibition activities of C. ovis toxin behaved identically to precipitation by ammonium sulphate, inactivation with formaldehyde and heat, neutralization by antitoxin and fractionation by Sephadex G-100. These two activities are considered to be the manifestation of the same protein moiety. The toxin appears to be composed of two components, one a stable high molecular weight and the other an unstable low molecular weight protein.
650 $aAnimal diseases
650 $aApplication methods
650 $aCaseous lymphadenitis
650 $aExotoxins
650 $aCorynebacterium Ovis
650 $aDoença Animal
650 $aLinfadenite Caseosa
653 $aAmmonium sulphate
653 $aCaracterização
700 1 $aSINGH, I. P.
773 $tJournal of Comparative Pathology$gv. 82, n. 3, p. 345-353, Jul. 1972.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
