01659naa a2200253 a 450000100080000000500110000800800410001902400540006010000160011424500720013026000090020252009080021165000200111965000240113965000260116365000140118965000250120365000190122865000240124765300220127165300210129370000170131477300740133115165322023-06-20       1972    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0021-9975(72)90016-32DOI1 aGOEL, M. C.  aPurification and characterization of Corynebacterium ovis exotoxin.  c1972  aAbstract: A scheme for the purification of Corynebacterium ovis toxin by fractional precipitation with ammonium sulphate has been described. Based on four experiments a mean yield of 219·1 per cent. of the original activity was obtained. The mean concentration effected was 96-fold. Expressing the activity of the toxin per mg. of non-dialysable nitrogen, the mean purification value achieved was 361·3 per cent. The dermo-necrotic and staphylococcal beta-haemolysin inhibition activities of C. ovis toxin behaved identically to precipitation by ammonium sulphate, inactivation with formaldehyde and heat, neutralization by antitoxin and fractionation by Sephadex G-100. These two activities are considered to be the manifestation of the same protein moiety. The toxin appears to be composed of two components, one a stable high molecular weight and the other an unstable low molecular weight protein.  aAnimal diseases  aApplication methods  aCaseous lymphadenitis  aExotoxins  aCorynebacterium Ovis  aDoença Animal  aLinfadenite Caseosa  aAmmonium sulphate  aCaracterização1 aSINGH, I. P.  tJournal of Comparative Pathologygv. 82, n. 3, p. 345-353, Jul. 1972.