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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/01/2006 |
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Data da última atualização: |
29/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALERIO, H. M.; RESENDE, M. A.; WEIKERT-OLIVEIRA, R. C. B.; CASELA, C. R. |
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Afiliação: |
Embrapa Milho e Sorgo. |
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Título: |
Virulence and molecular diversity in colletotrichum graminicola from Brazil. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Mycopathologia, The Hague, v. 159, n. 3, p. 449-459, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic diversity among 37 isolates of the sorghum anthracnose pathogen Colletotrichum graminicola, from four geographically distinct regions of Brazil, was evaluated by RAPD and RFLP-PCR markers and virulence characters on a set of 10 differential sorghum genotypes. Twenty-two races were identified and race 13B was the most frequent, but present in only two regions. RAPD analysis revealed 143 polymorphic bands that grouped the isolates according to their geographic origin, but not by their virulence phenotypes. RFLP with HaeIII, MspI, HinfI, HhaI, HpaII, EcoRI, HindlII, PstI, RsaI, Taq alpha I, and AluI enzymes over ITS domains and 5.8 rDNA genes of C. graminicola did not show differences among the isolates, indicating high conservation of these restriction sites. Molecular polymorphism was observed among isolates belonging to the same race. No association between virulence phenotypes and molecular profiles was observed. |
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Palavras-Chave: |
Diversity; RAPD; RFLP; Virulence phenotype. |
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Thesagro: |
Antracnose; Colletotrichum Graminicola; Sorghum bicolor. |
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Thesaurus Nal: |
polymorphism. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01656naa a2200253 a 4500
001 1489113
005 2018-05-29
008 2005 bl uuuu u00u1 u #d
100 1 $aVALERIO, H. M.
245 $aVirulence and molecular diversity in colletotrichum graminicola from Brazil.$h[electronic resource]
260 $c2005
520 $aGenetic diversity among 37 isolates of the sorghum anthracnose pathogen Colletotrichum graminicola, from four geographically distinct regions of Brazil, was evaluated by RAPD and RFLP-PCR markers and virulence characters on a set of 10 differential sorghum genotypes. Twenty-two races were identified and race 13B was the most frequent, but present in only two regions. RAPD analysis revealed 143 polymorphic bands that grouped the isolates according to their geographic origin, but not by their virulence phenotypes. RFLP with HaeIII, MspI, HinfI, HhaI, HpaII, EcoRI, HindlII, PstI, RsaI, Taq alpha I, and AluI enzymes over ITS domains and 5.8 rDNA genes of C. graminicola did not show differences among the isolates, indicating high conservation of these restriction sites. Molecular polymorphism was observed among isolates belonging to the same race. No association between virulence phenotypes and molecular profiles was observed.
650 $apolymorphism
650 $aAntracnose
650 $aColletotrichum Graminicola
650 $aSorghum bicolor
653 $aDiversity
653 $aRAPD
653 $aRFLP
653 $aVirulence phenotype
700 1 $aRESENDE, M. A.
700 1 $aWEIKERT-OLIVEIRA, R. C. B.
700 1 $aCASELA, C. R.
773 $tMycopathologia, The Hague$gv. 159, n. 3, p. 449-459, 2005.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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