01656naa a2200253 a 450000100080000000500110000800800410001910000190006024501040007926000090018352009400019265000170113265000150114965000310116465000200119565300140121565300090122965300090123865300240124770000190127170000310129070000180132177300630133914891132018-05-29       2005    bl uuuu     u00u1 u    #d1 aVALERIO, H. M.  aVirulence and molecular diversity in colletotrichum graminicola from Brazil.h[electronic resource]  c2005  aGenetic diversity among 37 isolates of the sorghum anthracnose pathogen Colletotrichum graminicola, from four geographically distinct regions of Brazil, was evaluated by RAPD and RFLP-PCR markers and virulence characters on a set of 10 differential sorghum genotypes. Twenty-two races were identified and race 13B was the most frequent, but present in only two regions. RAPD analysis revealed 143 polymorphic bands that grouped the isolates according to their geographic origin, but not by their virulence phenotypes. RFLP with HaeIII, MspI, HinfI, HhaI, HpaII, EcoRI, HindlII, PstI, RsaI, Taq alpha I, and AluI enzymes over ITS domains and 5.8 rDNA genes of C. graminicola did not show differences among the isolates, indicating high conservation of these restriction sites. Molecular polymorphism was observed among isolates belonging to the same race. No association between virulence phenotypes and molecular profiles was observed.  apolymorphism  aAntracnose  aColletotrichum Graminicola  aSorghum bicolor  aDiversity  aRAPD  aRFLP  aVirulence phenotype1 aRESENDE, M. A.1 aWEIKERT-OLIVEIRA, R. C. B.1 aCASELA, C. R.  tMycopathologia, The Haguegv. 159, n. 3, p. 449-459, 2005.