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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/06/2017 |
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Data da última atualização: |
23/06/2017 |
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Autoria: |
CAMPBELL, B. K.; MANN, G. E.; MCNEILLY, A. S.; BAIRD, D. T. |
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Título: |
Pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary of the sheep during the oestrous cycle. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Journal of Endocrinology, v. 126, n. 3, p. 385-393, Sep. 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The pattern of pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary at different stages of the oestrous cycle in sheep was studied in five Finn-Merino ewes in which the left ovary had been auto-transplanted to the neck. The ewes had jugular venous blood samples collected at 4-hourly intervals from 42 h before the induction of luteolysis by i.m. injection of cloprostenol (100 micrograms) on day 10 of the oestrous cycle, until day 3 of the following cycle. There were five periods of intensive blood sampling, when both ovarian and jugular venous blood samples were collected, as follows: (a) mid-luteal phase, before the second injection of cloprostenol on day 10 (15-min intervals for 6 h); (b) early follicular phase, 24 h after the second injection of cloprostenol (10-min intervals for 4 h); (c) late follicular phase, 48 h after the second injection of cloprostenol (10-min intervals for 4 h); (d) after the LH surge on day 1 of the cycle, 76 h after the second injection of cloprostenol (10-min intervals for 4 h); (e) early luteal phase on day 3 of the cycle, 120 h after the second injection of cloprostenol (10-min intervals for 3 h). Plasma was collected and the samples assayed for LH, FSH, progesterone, oestradiol, androstenedione and inhibin. The ovarian secretion rates for oestradiol, androstenedione and inhibin were calculated. All ewes responded normally to the luteolytic dose of cloprostenol with the preovulatory surge of LH occurring within 56.4 +/- 1.6 h (mean +/- S.E.M.) followed by the establishment of a normal luteal phase. MenosAbstract: The pattern of pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary at different stages of the oestrous cycle in sheep was studied in five Finn-Merino ewes in which the left ovary had been auto-transplanted to the neck. The ewes had jugular venous blood samples collected at 4-hourly intervals from 42 h before the induction of luteolysis by i.m. injection of cloprostenol (100 micrograms) on day 10 of the oestrous cycle, until day 3 of the following cycle. There were five periods of intensive blood sampling, when both ovarian and jugular venous blood samples were collected, as follows: (a) mid-luteal phase, before the second injection of cloprostenol on day 10 (15-min intervals for 6 h); (b) early follicular phase, 24 h after the second injection of cloprostenol (10-min intervals for 4 h); (c) late follicular phase, 48 h after the second injection of cloprostenol (10-min intervals for 4 h); (d) after the LH surge on day 1 of the cycle, 76 h after the second injection of cloprostenol (10-min intervals for 4 h); (e) early luteal phase on day 3 of the cycle, 120 h after the second injection of cloprostenol (10-min intervals for 3 h). Plasma was collected and the samples assayed for LH, FSH, progesterone, oestradiol, androstenedione and inhibin. The ovarian secretion rates for oestradiol, androstenedione and inhibin were calculated. All ewes responded normally to the luteolytic dose of cloprostenol with the preovulatory surge of LH occurring within... Mostrar Tudo |
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Palavras-Chave: |
FSH; Gonadal Steroid Hormone; Inhibins; LH. |
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Thesagro: |
Estradiol. |
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Thesaurus Nal: |
Androstenedione; Physiology; Pituitary Gland; Prolactin; Secretion; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02375naa a2200289 a 4500
001 2071399
005 2017-06-23
008 1990 bl uuuu u00u1 u #d
100 1 $aCAMPBELL, B. K.
245 $aPulsatile secretion of inhibin, oestradiol and androstenedione by the ovary of the sheep during the oestrous cycle.
260 $c1990
520 $aAbstract: The pattern of pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary at different stages of the oestrous cycle in sheep was studied in five Finn-Merino ewes in which the left ovary had been auto-transplanted to the neck. The ewes had jugular venous blood samples collected at 4-hourly intervals from 42 h before the induction of luteolysis by i.m. injection of cloprostenol (100 micrograms) on day 10 of the oestrous cycle, until day 3 of the following cycle. There were five periods of intensive blood sampling, when both ovarian and jugular venous blood samples were collected, as follows: (a) mid-luteal phase, before the second injection of cloprostenol on day 10 (15-min intervals for 6 h); (b) early follicular phase, 24 h after the second injection of cloprostenol (10-min intervals for 4 h); (c) late follicular phase, 48 h after the second injection of cloprostenol (10-min intervals for 4 h); (d) after the LH surge on day 1 of the cycle, 76 h after the second injection of cloprostenol (10-min intervals for 4 h); (e) early luteal phase on day 3 of the cycle, 120 h after the second injection of cloprostenol (10-min intervals for 3 h). Plasma was collected and the samples assayed for LH, FSH, progesterone, oestradiol, androstenedione and inhibin. The ovarian secretion rates for oestradiol, androstenedione and inhibin were calculated. All ewes responded normally to the luteolytic dose of cloprostenol with the preovulatory surge of LH occurring within 56.4 +/- 1.6 h (mean +/- S.E.M.) followed by the establishment of a normal luteal phase.
650 $aAndrostenedione
650 $aPhysiology
650 $aPituitary Gland
650 $aProlactin
650 $aSecretion
650 $aSheep
650 $aEstradiol
653 $aFSH
653 $aGonadal Steroid Hormone
653 $aInhibins
653 $aLH
700 1 $aMANN, G. E.
700 1 $aMCNEILLY, A. S.
700 1 $aBAIRD, D. T.
773 $tJournal of Endocrinology$gv. 126, n. 3, p. 385-393, Sep. 1990.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/11/2020 |
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Data da última atualização: |
23/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GRUNENNVALDT, R. L.; DEGENHARDT, J.; BROOKS, P.; TOMASI, J. de C.; HANSEL, F. A.; TRAN, T.; GOMES, E. N.; DESCHAMPS, C. |
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Afiliação: |
RENATA LÚCIA GRUNENNVALDT, UFPR; JULIANA DEGENHARDT, CNPF; PETER BROOKS, University of The Sunshine Coast; JÉSSICA DE CÁSSIA TOMASI, UFPR; FABRICIO AUGUSTO HANSEL, CNPF; TRONG TRAN, University of The Sunshine Coast; ERIK N. GOMES, The State University of New Jersey; CÍCERO DESCHAMPS, UFPR. |
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Título: |
Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p. |
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DOI: |
https://doi.org/10.1590/0001-3765202020181251 |
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Idioma: |
Inglês |
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Conteúdo: |
Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine. |
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Palavras-Chave: |
Acúmulo de biomassa; Atividade antioxidante; Biomass accumulation; Erva mate; Plant growth regulators. |
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Thesagro: |
Ilex Paraguariensis; Regulador de Crescimento; Sacarose. |
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Thesaurus NAL: |
Antioxidant activity; Sucrose; Yerba mate. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217736/1/Juliana-Grunennvaldt-et-al-2020.pdf
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Marc: |
LEADER 02511naa a2200349 a 4500
001 2126527
005 2020-11-23
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0001-3765202020181251$2DOI
100 1 $aGRUNENNVALDT, R. L.
245 $aCallus culture as a new approach for the production of high added value compounds in Ilex paraguariensis$bgenotype infl uence, medium optimization and compounds identifi cation.$h[electronic resource]
260 $c2020
520 $aIlex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
650 $aAntioxidant activity
650 $aSucrose
650 $aYerba mate
650 $aIlex Paraguariensis
650 $aRegulador de Crescimento
650 $aSacarose
653 $aAcúmulo de biomassa
653 $aAtividade antioxidante
653 $aBiomass accumulation
653 $aErva mate
653 $aPlant growth regulators
700 1 $aDEGENHARDT, J.
700 1 $aBROOKS, P.
700 1 $aTOMASI, J. de C.
700 1 $aHANSEL, F. A.
700 1 $aTRAN, T.
700 1 $aGOMES, E. N.
700 1 $aDESCHAMPS, C.
773 $tAnais da Academia Brasileira de Ciências$gv. 92, n. 3, e20181251, 2020. 16 p.
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Embrapa Florestas (CNPF) |
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