02375naa a2200289 a 450000100080000000500110000800800410001910000200006024501200008026000090020052015930020965000200180265000150182265000200183765000140185765000140187165000100188565000140189565300080190965300280191765300130194565300070195870000160196570000200198170000170200177300670201820713992017-06-23       1990    bl uuuu     u00u1 u    #d1 aCAMPBELL, B. K.  aPulsatile secretion of inhibin, oestradiol and androstenedione by the ovary of the sheep during the oestrous cycle.  c1990  aAbstract: The pattern of pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary at different stages of the oestrous cycle in sheep was studied in five Finn-Merino ewes in which the left ovary had been auto-transplanted to the neck. The ewes had jugular venous blood samples collected at 4-hourly intervals from 42 h before the induction of luteolysis by i.m. injection of cloprostenol (100 micrograms) on day 10 of the oestrous cycle, until day 3 of the following cycle. There were five periods of intensive blood sampling, when both ovarian and jugular venous blood samples were collected, as follows: (a) mid-luteal phase, before the second injection of cloprostenol on day 10 (15-min intervals for 6 h); (b) early follicular phase, 24 h after the second injection of cloprostenol (10-min intervals for 4 h); (c) late follicular phase, 48 h after the second injection of cloprostenol (10-min intervals for 4 h); (d) after the LH surge on day 1 of the cycle, 76 h after the second injection of cloprostenol (10-min intervals for 4 h); (e) early luteal phase on day 3 of the cycle, 120 h after the second injection of cloprostenol (10-min intervals for 3 h). Plasma was collected and the samples assayed for LH, FSH, progesterone, oestradiol, androstenedione and inhibin. The ovarian secretion rates for oestradiol, androstenedione and inhibin were calculated. All ewes responded normally to the luteolytic dose of cloprostenol with the preovulatory surge of LH occurring within 56.4 +/- 1.6 h (mean +/- S.E.M.) followed by the establishment of a normal luteal phase.  aAndrostenedione  aPhysiology  aPituitary Gland  aProlactin  aSecretion  aSheep  aEstradiol  aFSH  aGonadal Steroid Hormone  aInhibins  aLH1 aMANN, G. E.1 aMCNEILLY, A. S.1 aBAIRD, D. T.  tJournal of Endocrinologygv. 126, n. 3, p. 385-393, Sep. 1990.