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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/04/2021 |
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Data da última atualização: |
12/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
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Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
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Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
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ISSN: |
1874-3919 |
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DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
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Idioma: |
Inglês
Português |
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Notas: |
Na publicação: Angela Mehta. |
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Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
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Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
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Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
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Thesaurus Nal: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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Marc: |
LEADER 03484naa a2200469 a 4500
001 2131497
005 2021-07-12
008 2021 bl uuuu u00u1 u #d
022 $a1874-3919
024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Angela Mehta.
520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance.
650 $ablast disease
650 $adisease resistance
650 $afoliar diseases
650 $aMagnaporthe oryzae
650 $aplant diseases and disorders
650 $aproteomics
650 $arice
650 $aArroz
650 $aBrusone
650 $aDoença de Planta
650 $aOryza Sativa
653 $aGene ontology
653 $aPlant immunity
653 $aPTO-based TIGS
653 $aRT-qPCR
653 $aS-gene
653 $aTransient-Induced gene silencing
700 1 $aBEVITORI, R.
700 1 $aMELLO, R. N. de
700 1 $aCINTRA, M. M. D. F.
700 1 $aOLIVEIRA NETO, O. B.
700 1 $aFONTES, W.
700 1 $aCASTRO, M. S.
700 1 $aSOUSA, M. V.
700 1 $aFRANCO, O. L.
700 1 $aREIS, A. M. dos
773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/07/2019 |
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Data da última atualização: |
07/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WILCKEN, C. F.; DAL POGETTO, M. H. F. do A.; LIMA, A. C. V.; SOLIMAN, E. P.; FERNANDES, B. V.; SILVA, I. M. da; ZANUNCIO, A. J. V.; BARBOSA, L. R.; ZANUNCIO, J. C. |
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Afiliação: |
Carlos Frederico Wilcken, UNESP; Mário Henrique Ferreira do Amaral Dal Pogetto, Dow Agrosciences; Alexandre Coutinho Vianna Lima, MIP Florestal; Everton Pires Soliman, UNESP; Bianca Vique Fernandes, JB Biotecnologia; Isabel Moreira da Silva, UFV; Antonio José Vinha Zanuncio, UFV; LEONARDO RODRIGUES BARBOSA, CNPF; José Cola Zanuncio, BIOAGRO, UFV. |
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Título: |
Chemical vs entomopathogenic control of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) via aerial application in eucalyptus plantations. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientific Reports, v. 9, article 9416, 2019. 7 p. |
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DOI: |
10.1038/s41598-019-45802-y |
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Idioma: |
Inglês |
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Conteúdo: |
The Thaumastocoris peregrinus spread to eucalyptus plantations in many countries. Chemical control is a questionable measure, mainly due to the environmental impact, high cost and moreover has the use restricted by the forest certifcations. Bio-insecticides may have similar efciency to chemical products to control T. peregrinus. The chemical thiamethoxam, thiamethoxam+lambda-cyhalothrin, acephate and the microbial Beauveria bassiana and Metarhizium anisopliae insecticides were tested at diferent doses to manage T. peregrinus. The products were sprayed on eucalyptus plants using aircraft and populations of this insect were counted before application and at 1, 14 and 21days afterwards (DAA). Ten eucalyptus trees were evaluated per plot, with the collection of ten leaves from the middle third of the crown of each tree, and the number of T. peregrinus nymphs and adults obtained per leaf was determined. All the chemical insecticides had similar control at 1 DAA for T. peregrinus nymphs and adults. At 14 DAA, the number of T. peregrinus nymphs and adults on eucalyptus leaves was similar for the chemical and microbial insecticide treatments. At 21 DAA the control efciency of T. peregrinus nymphs and adults was higher than 80% with all insecticides. The entomopathogenic insecticides have potential for aerial application to control T. peregrinus nymphs and adults and provide viable and environmentally-friendly alternative to manage this pest. |
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Palavras-Chave: |
Controle; Percevejo bronzeado; Praga florestal; Thaumastocoris peregrinus. |
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Thesagro: |
Eucalipto. |
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Thesaurus NAL: |
Eucalyptus; Pest control. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199072/1/2019-Leonardo-SR-Chemical.pdf
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Marc: |
LEADER 02434naa a2200313 a 4500
001 2110315
005 2019-11-07
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1038/s41598-019-45802-y$2DOI
100 1 $aWILCKEN, C. F.
245 $aChemical vs entomopathogenic control of Thaumastocoris peregrinus (Hemiptera$bThaumastocoridae) via aerial application in eucalyptus plantations.$h[electronic resource]
260 $c2019
520 $aThe Thaumastocoris peregrinus spread to eucalyptus plantations in many countries. Chemical control is a questionable measure, mainly due to the environmental impact, high cost and moreover has the use restricted by the forest certifcations. Bio-insecticides may have similar efciency to chemical products to control T. peregrinus. The chemical thiamethoxam, thiamethoxam+lambda-cyhalothrin, acephate and the microbial Beauveria bassiana and Metarhizium anisopliae insecticides were tested at diferent doses to manage T. peregrinus. The products were sprayed on eucalyptus plants using aircraft and populations of this insect were counted before application and at 1, 14 and 21days afterwards (DAA). Ten eucalyptus trees were evaluated per plot, with the collection of ten leaves from the middle third of the crown of each tree, and the number of T. peregrinus nymphs and adults obtained per leaf was determined. All the chemical insecticides had similar control at 1 DAA for T. peregrinus nymphs and adults. At 14 DAA, the number of T. peregrinus nymphs and adults on eucalyptus leaves was similar for the chemical and microbial insecticide treatments. At 21 DAA the control efciency of T. peregrinus nymphs and adults was higher than 80% with all insecticides. The entomopathogenic insecticides have potential for aerial application to control T. peregrinus nymphs and adults and provide viable and environmentally-friendly alternative to manage this pest.
650 $aEucalyptus
650 $aPest control
650 $aEucalipto
653 $aControle
653 $aPercevejo bronzeado
653 $aPraga florestal
653 $aThaumastocoris peregrinus
700 1 $aDAL POGETTO, M. H. F. do A.
700 1 $aLIMA, A. C. V.
700 1 $aSOLIMAN, E. P.
700 1 $aFERNANDES, B. V.
700 1 $aSILVA, I. M. da
700 1 $aZANUNCIO, A. J. V.
700 1 $aBARBOSA, L. R.
700 1 $aZANUNCIO, J. C.
773 $tScientific Reports$gv. 9, article 9416, 2019. 7 p.
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