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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
MARTINS, F. S.; SARAIVA, M. V. A.; CELESTINO, J. J. H.; BRUNO, J. B.; ALMEIDA, A. P.; CUNHA, R. M. S.; SILVA, J. R. V.; CAMPELO, C. C.; LUCCI, C. M.; MATOS, M. H. T.; FIGUEIREDO, J. R. |
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Título: |
Expression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Animal Reproduction, v. 7, n. 4, p. 349-361, Oct./Dec. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles. MenosAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed ... Mostrar Tudo |
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Palavras-Chave: |
Folículo pré-antral; IGF-I. |
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Thesagro: |
Cabra; Caprino; Endocrinologia; Fator de crescimento; Hormônio; Insulina; Ovário; Reprodução animal. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 4 | |
| 1. |  | AMABILE, R. F.; FIDELIS, L. R. G.; INÁCIO, A. A. do N.; SOUSA, D. A.; MONTEIRO, V. A.; PEREIRA, V. C.; GOMES, A. C. Estudo do efeito de épocas de semeadura e influência do posicionamento dos grãos em espiga na germinação de sementes de cevada (Hordeum vulgare L.) na região do cerrado. In: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 27., 2009, Passo Fundo. Anais... Passo Fundo: Embrapa Trigo, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
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| 2. | | AMABILE, R. F.; FIDELIS, L. R. G.; INÁCIO, A. A. do N.; SOUSA, D. A.; MONTEIRO, V. A.; GOMES, A. C. Estudo do efeito de épocas de semeadura e influência do posicionamento dos grãos em espiga na germinação de sementes de cevada (Hordeum vulgare) na região do cerrado. In: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 26., 2007, Passo Fundo. Anais. Passo Fundo: Embrapa Trigo, 2007. p. 342-350. (Embrapa Trigo. Documentos, 76).| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Cerrados. |
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| 3. | | SOUSA, D. A.; CASCON, C. M.; MELLO, M. F. V.; LEITE, J. S.; MEDEIROS, M. A.; FONSECA, A. B. M.; FERREIRA, A. M. R. Helicobacter spp. in domestic cats: identification and relationship with anatomical and histopathological gastric changes in animals of blood group A. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1467-1473, dezembro 2017. Título em português: Helicobacter spp. em gatos domésticos: identificação e relação com alterações gástricas anatômicas e histopatológicas em animais de sangue tipo A.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | NETO, S. M.; COSTA, F. T.; ALMEIDA, R. G.; SOUSA, D. A.; BLOCH JÚNIOR, C. VASCONCELOS, I. M.; OLIVEIRA, J. T.; FRANCO, O. L. Isolation and molecular characterization of a novel 2S albumin with antimicrobial properties from Sesamum indicum L. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 4 | |
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