| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
07/05/2013 |
|
Data da última atualização: |
17/01/2024 |
|
Autoria: |
MARTINS, F. S.; SARAIVA, M. V. A.; CELESTINO, J. J. H.; BRUNO, J. B.; ALMEIDA, A. P.; CUNHA, R. M. S.; SILVA, J. R. V.; CAMPELO, C. C.; LUCCI, C. M.; MATOS, M. H. T.; FIGUEIREDO, J. R. |
|
Título: |
Expression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Animal Reproduction, v. 7, n. 4, p. 349-361, Oct./Dec. 2010. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles. MenosAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed ... Mostrar Tudo |
|
Palavras-Chave: |
Folículo pré-antral; IGF-I. |
|
Thesagro: |
Cabra; Caprino; Endocrinologia; Fator de crescimento; Hormônio; Insulina; Ovário; Reprodução animal. |
|
Categoria do assunto: |
-- |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | STORCH, E. R.; JUNQUEIRA, N. T. V.; RANGEL, L. E. P.; SANTOS, G. R. do; NASCIMENTO, A. C. do; FIALHO, J. de F.; KOSOSKI, R. M.; SILVA, J. A. da. Potencial de alguns defensivos agrícolas naturais para o controle de doenças de mamão papaya na pós-colheita. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 1., 2000, Fortaleza. Anais... Fortaleza: Academia Cearense de Ciências, 2000. 1 p. Título em inglês: Potential of some natural pesticides to control of post-harvest diseases on papaya.| Biblioteca(s): Embrapa Cerrados. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
