02813naa a2200361 a 450000100080000000500110000800800410001910000190006024502210007926000090030052017120030965000100202165000120203165000190204365000250206265000140208765000130210165000120211465000240212665300260215065300100217670000220218670000240220870000170223270000190224970000200226870000200228870000190230870000170232770000200234470000220236477300650238619572412024-01-17       2010    bl uuuu     u00u1 u    #d1 aMARTINS, F. S.  aExpression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles.h[electronic resource]  c2010  aAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P &lt 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles.  aCabra  aCaprino  aEndocrinologia  aFator de crescimento  aHormônio  aInsulina  aOvário  aReprodução animal  aFolículo pré-antral  aIGF-I1 aSARAIVA, M. V. A.1 aCELESTINO, J. J. H.1 aBRUNO, J. B.1 aALMEIDA, A. P.1 aCUNHA, R. M. S.1 aSILVA, J. R. V.1 aCAMPELO, C. C.1 aLUCCI, C. M.1 aMATOS, M. H. T.1 aFIGUEIREDO, J. R.  tAnimal Reproductiongv. 7, n. 4, p. 349-361, Oct./Dec. 2010.