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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
29/03/2016 |
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Autoria: |
BARTON, K. A.; WHITELEY, H. R.; YANG, N. S. |
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Título: |
Bacillus thuringiensis endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Plant Physiology, v. 85, p. 1103-1109, 1987. |
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Idioma: |
Inglês |
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Conteúdo: |
The crystal proteins, or 5-endotoxins, of Bacillus thuringiensis are specifically lethal to Lepidopteran insects. We utilized a truncated and modified portion of a cloned crystal protein gene to construct a chimeric gene capable of expression in plant cells. Using an Agrobacterium tumefaciens binary vector system, we then transferred the chimeric toxin gene into tobacco (Nicotiana tabacum cv Havana 425) cells and regenerated recombinant plants. One to several copies per cell of the toxin gene are routinely present in the recombinant plants. Hybridization experiments demonstrated that these plants had a new RNA species of the size expected for the truncated toxin mRNA, and a polypeptide having the mobility expected for the truncated toxin was detected by immunoblotting. Significant variation was found in the levels of toxin-specific RNA expression between different recombinants, but the levels of hybridizing RNA in transformants correlated with the level of toxicity demonstrated against Manduca sexta (tobacco hornworm), and other Lepidopteran insects. The recombinant genes were transmitted to progeny and resistance to insects was maintained, thus demonstrating that the introduction of toxin genes into plants may be a practical method of providing protection against certain insect pests. |
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Thesagro: |
Controle Biológico. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01789naa a2200157 a 4500
001 1837899
005 2016-03-29
008 1987 bl --- 0-- u #d
100 1 $aBARTON, K. A.
245 $aBacillus thuringiensis endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects.
260 $c1987
520 $aThe crystal proteins, or 5-endotoxins, of Bacillus thuringiensis are specifically lethal to Lepidopteran insects. We utilized a truncated and modified portion of a cloned crystal protein gene to construct a chimeric gene capable of expression in plant cells. Using an Agrobacterium tumefaciens binary vector system, we then transferred the chimeric toxin gene into tobacco (Nicotiana tabacum cv Havana 425) cells and regenerated recombinant plants. One to several copies per cell of the toxin gene are routinely present in the recombinant plants. Hybridization experiments demonstrated that these plants had a new RNA species of the size expected for the truncated toxin mRNA, and a polypeptide having the mobility expected for the truncated toxin was detected by immunoblotting. Significant variation was found in the levels of toxin-specific RNA expression between different recombinants, but the levels of hybridizing RNA in transformants correlated with the level of toxicity demonstrated against Manduca sexta (tobacco hornworm), and other Lepidopteran insects. The recombinant genes were transmitted to progeny and resistance to insects was maintained, thus demonstrating that the introduction of toxin genes into plants may be a practical method of providing protection against certain insect pests.
650 $aControle Biológico
700 1 $aWHITELEY, H. R.
700 1 $aYANG, N. S.
773 $tPlant Physiology$gv. 85, p. 1103-1109, 1987.
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Registro original: |
Embrapa Trigo (CNPT) |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
25/11/2021 |
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Data da última atualização: |
25/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, M. C. da; COSTA, I. C.; NAKAZATO, G.; PINHEIRO, J. W.; LEDUR, M. C.; LOYOLA, W.; VENANCIO, E. J. |
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Afiliação: |
MIRIELE CAROLINE DA SILVA, UEL; IVETE CONCHON COSTA, UEL; GERSON NAKAZATO, UEL; JOÃO WAINE PINHEIRO, UEL; MONICA CORREA LEDUR, CNPSA; WAGNER LOYOLA, CNPSA; EMERSON JOSÉ VENANCIO, UEL. |
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Título: |
Innate immunity in chicken lines developed by Embrapa Suínos e Aves: antimicrobial activity of macrophages and serum. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 50, n. e20200229, 2021. |
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DOI: |
https://doi.org/10.37496/rbz5020200229 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective of this study was to compare the antimicrobial activity of macrophages and serum in laying hen (MM, CC, and CCc) and broiler chicken lineages (TT and LL). Macrophages were evaluated for phagocytic and antimicrobial activity. Microbicidal serum activity was evaluated by the resistance test for serum and the agar test. The results showed that phagocytic activity was higher in males of the MM strain, with 13% of macrophages presenting phagocytosis, while the other lineages studied, and even female MM, presented a rate of 6% of phagocytic cells. However, antimicrobial activity in macrophages from males of CCc lineage and females of TT lineage were higher, eliminating more than 30% of the Salmonella enterica inoculum, while in the other strains, the results were similar, with inoculum reduction below 30%. In the serum resistance assay, female laying lines presented higher antibacterial activity than female broiler lines. In the trials to evaluate the microbicide activity of the serum, females of both broiler and laying lineages presented higher performance when compared with males of the same lineage. Females of laying hen lines (MM and CC) present a greater antibacterium activity than males. These results can contribute to a better understanding of the immune response in broiler chicken and laying hen lineages, to aid development of lineages of birds more resistant to pathogens |
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Palavras-Chave: |
Complement system; Fowls; Genetic selection; Imunidade natural; Natural immunity. |
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Thesagro: |
Ave Domestica; Frango de Corte; Galinha Para Postura; Genética Animal; Poedeira Comercial; Seleção Genética. |
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Thesaurus NAL: |
Broiler chickens; Laying hens. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1136555/1/final9220.pdf
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Marc: |
LEADER 02505naa a2200361 a 4500
001 2136555
005 2021-11-25
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.37496/rbz5020200229$2DOI
100 1 $aSILVA, M. C. da
245 $aInnate immunity in chicken lines developed by Embrapa Suínos e Aves$bantimicrobial activity of macrophages and serum.$h[electronic resource]
260 $c2021
520 $aAbstract: The objective of this study was to compare the antimicrobial activity of macrophages and serum in laying hen (MM, CC, and CCc) and broiler chicken lineages (TT and LL). Macrophages were evaluated for phagocytic and antimicrobial activity. Microbicidal serum activity was evaluated by the resistance test for serum and the agar test. The results showed that phagocytic activity was higher in males of the MM strain, with 13% of macrophages presenting phagocytosis, while the other lineages studied, and even female MM, presented a rate of 6% of phagocytic cells. However, antimicrobial activity in macrophages from males of CCc lineage and females of TT lineage were higher, eliminating more than 30% of the Salmonella enterica inoculum, while in the other strains, the results were similar, with inoculum reduction below 30%. In the serum resistance assay, female laying lines presented higher antibacterial activity than female broiler lines. In the trials to evaluate the microbicide activity of the serum, females of both broiler and laying lineages presented higher performance when compared with males of the same lineage. Females of laying hen lines (MM and CC) present a greater antibacterium activity than males. These results can contribute to a better understanding of the immune response in broiler chicken and laying hen lineages, to aid development of lineages of birds more resistant to pathogens
650 $aBroiler chickens
650 $aLaying hens
650 $aAve Domestica
650 $aFrango de Corte
650 $aGalinha Para Postura
650 $aGenética Animal
650 $aPoedeira Comercial
650 $aSeleção Genética
653 $aComplement system
653 $aFowls
653 $aGenetic selection
653 $aImunidade natural
653 $aNatural immunity
700 1 $aCOSTA, I. C.
700 1 $aNAKAZATO, G.
700 1 $aPINHEIRO, J. W.
700 1 $aLEDUR, M. C.
700 1 $aLOYOLA, W.
700 1 $aVENANCIO, E. J.
773 $tRevista Brasileira de Zootecnia$gv. 50, n. e20200229, 2021.
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