Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
29/11/1999 |
Data da última atualização: |
15/05/2023 |
Autoria: |
AMIN, M. N.; JAISWAL, V. S. |
Afiliação: |
Department of Botani, University of Rajshahi, Rajshahi, Bangladesh; Department of Botany, Banaras Hindu University, Varanasi - 221 005, India. |
Título: |
In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus). |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.33, p.59-65, 1993. |
Idioma: |
Inglês |
Conteúdo: |
A tissue culture technique for rapid vegetative propagation of mature jackfruit trees using apical bud cultures has been developed. Shoot-tip cultures were established on MS medium with 5-10mm explants dissected from terminal buds of new growth from trunk. After initial culture of bud explants, one-to two-node pieces were taken from the microshoots formed and used to proliferate further axillary shoots for multiplying and maintaining shoot cultures. Benzyladenine and kinetin (4.5-9.0 uM), either separately or together, supported shoot proliferation; higher concentrations of the cytokinins inhibited bud breaking and favoured callus formation at the explant bases. Bud explants taken from emerging trunk sprouts invariably produced clumps of multiple shoots, whereas buds obtained from actively growing top branches generally elongated to form a solitary shoot. |
Palavras-Chave: |
Apical buds; Cultura de tecidos; Gemas apical; Proliferacao de apices; Shoot proliferation. |
Thesagro: |
Enraizamento; Micropropagação. |
Thesaurus Nal: |
micropropagation; rooting; tissue culture. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01618naa a2200253 a 4500 001 1648441 005 2023-05-15 008 1993 bl uuuu u00u1 u #d 100 1 $aAMIN, M. N. 245 $aIn vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus).$h[electronic resource] 260 $c1993 520 $aA tissue culture technique for rapid vegetative propagation of mature jackfruit trees using apical bud cultures has been developed. Shoot-tip cultures were established on MS medium with 5-10mm explants dissected from terminal buds of new growth from trunk. After initial culture of bud explants, one-to two-node pieces were taken from the microshoots formed and used to proliferate further axillary shoots for multiplying and maintaining shoot cultures. Benzyladenine and kinetin (4.5-9.0 uM), either separately or together, supported shoot proliferation; higher concentrations of the cytokinins inhibited bud breaking and favoured callus formation at the explant bases. Bud explants taken from emerging trunk sprouts invariably produced clumps of multiple shoots, whereas buds obtained from actively growing top branches generally elongated to form a solitary shoot. 650 $amicropropagation 650 $arooting 650 $atissue culture 650 $aEnraizamento 650 $aMicropropagação 653 $aApical buds 653 $aCultura de tecidos 653 $aGemas apical 653 $aProliferacao de apices 653 $aShoot proliferation 700 1 $aJAISWAL, V. S. 773 $tPlant Cell, Tissue and Organ Culture$gv.33, p.59-65, 1993.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |