01618naa a2200253 a 450000100080000000500110000800800410001910000160006024501270007626000090020352008720021265000210108465000120110565000190111765000170113665000220115365300160117565300230119165300170121465300270123165300240125870000190128277300630130116484412023-05-15       1993    bl uuuu     u00u1 u    #d1 aAMIN, M. N.  aIn vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus).h[electronic resource]  c1993  aA tissue culture technique for rapid vegetative propagation of mature jackfruit trees using apical bud cultures has been developed. Shoot-tip cultures were established on MS medium with 5-10mm explants dissected from terminal buds of new growth from trunk. After initial culture of bud explants, one-to two-node pieces were taken from the microshoots formed and used to proliferate further axillary shoots for multiplying and maintaining shoot cultures. Benzyladenine and kinetin (4.5-9.0 uM), either separately or together, supported shoot proliferation; higher concentrations of the cytokinins inhibited bud breaking and favoured callus formation at the explant bases. Bud explants taken from emerging trunk sprouts invariably produced clumps of multiple shoots, whereas buds obtained from actively growing top branches generally elongated to form a solitary shoot.  amicropropagation  arooting  atissue culture  aEnraizamento  aMicropropagação  aApical buds  aCultura de tecidos  aGemas apical  aProliferacao de apices  aShoot proliferation1 aJAISWAL, V. S.  tPlant Cell, Tissue and Organ Culturegv.33, p.59-65, 1993.