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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/11/1999 |
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Data da última atualização: |
15/05/2023 |
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Autoria: |
SIM, G-E.; LOH, C-S.; GOH, C-J. |
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Afiliação: |
Tissue Culture Laboratory, Botany Department, National University of Singapore, Lower Kent Ridge Road, Singapore 0511, Republic of Singapore. |
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Título: |
Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Plant Cell Reports, v.7, p.418-420, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2 x 10 6/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1mg1-1 GA3, they developed into plantlets. Rooted plantlets were obtained in 3 months after protoplast isolation. |
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Palavras-Chave: |
Citrus mitis; Embrioes somaticos; Protoplastos; Regeneracao de plantula; Regeneration of plantlets. |
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Thesagro: |
Cultura de Tecido. |
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Thesaurus Nal: |
protoplasts; somatic embryos; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01221naa a2200253 a 4500
001 1648417
005 2023-05-15
008 1988 bl uuuu u00u1 u #d
100 1 $aSIM, G-E.
245 $aDirect somatic embryogenesis from protoplasts of Citrus mitis Blanco.$h[electronic resource]
260 $c1988
520 $aProtoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2 x 10 6/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1mg1-1 GA3, they developed into plantlets. Rooted plantlets were obtained in 3 months after protoplast isolation.
650 $aprotoplasts
650 $asomatic embryos
650 $atissue culture
650 $aCultura de Tecido
653 $aCitrus mitis
653 $aEmbrioes somaticos
653 $aProtoplastos
653 $aRegeneracao de plantula
653 $aRegeneration of plantlets
700 1 $aLOH, C-S.
700 1 $aGOH, C-J.
773 $tPlant Cell Reports$gv.7, p.418-420, 1988.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
