01221naa a2200253 a 450000100080000000500110000800800410001910000140006024500970007426000090017152005210018065000160070165000200071765000190073765000220075665300170077865300230079565300170081865300280083565300300086370000140089370000140090777300460092116484172023-05-15       1988    bl uuuu     u00u1 u    #d1 aSIM, G-E.  aDirect somatic embryogenesis from protoplasts of Citrus mitis Blanco.h[electronic resource]  c1988  aProtoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2 x 10 6/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1mg1-1 GA3, they developed into plantlets. Rooted plantlets were obtained in 3 months after protoplast isolation.  aprotoplasts  asomatic embryos  atissue culture  aCultura de Tecido  aCitrus mitis  aEmbrioes somaticos  aProtoplastos  aRegeneracao de plantula  aRegeneration of plantlets1 aLOH, C-S.1 aGOH, C-J.  tPlant Cell Reportsgv.7, p.418-420, 1988.