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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/09/1997 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
Niedz, R. P. |
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Título: |
Culturing embryogenic protoplasts of 'Hamlin' sweet orange in calcium alginate beads. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.34, n.1, p.19-25, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
Protoplasts were enzymatically isolated from log embryogenic sweet orang [Citrus sinensis (L.) Osbeck cv. Hamlin] suspension cultures embedded in 3-mm diameter Ca-alginate beads, and cultured in a growth cabinet (15-20u mol m-2 s-1, 4-h photoperiod, 27oC). Plating efficiency exceeded 90% for Ca-alginate embedded protoplasts vs. 30% for protoplasts cultured in a liquid medium. Embryoids formed from protoplasts were recoverd after 20 days by dissolving the Ca-alginate matrix with a calcium sequestrant. embryoids readily formed shoots that were rooted on MS + 0.0luM NAA + 5% sucrose. Potential applications are discussed. |
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Palavras-Chave: |
Alginate; Alginato; Calciun; Embriogenese Somatic; Embriogenese Somatica; Protoplast; Protoplastos. |
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Thesagro: |
Cálcio. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01270naa a2200229 a 4500
001 1647989
005 2023-05-17
008 1993 bl uuuu u00u1 u #d
100 1 $aNiedz, R. P
245 $aCulturing embryogenic protoplasts of 'Hamlin' sweet orange in calcium alginate beads.$h[electronic resource]
260 $c1993
520 $aProtoplasts were enzymatically isolated from log embryogenic sweet orang [Citrus sinensis (L.) Osbeck cv. Hamlin] suspension cultures embedded in 3-mm diameter Ca-alginate beads, and cultured in a growth cabinet (15-20u mol m-2 s-1, 4-h photoperiod, 27oC). Plating efficiency exceeded 90% for Ca-alginate embedded protoplasts vs. 30% for protoplasts cultured in a liquid medium. Embryoids formed from protoplasts were recoverd after 20 days by dissolving the Ca-alginate matrix with a calcium sequestrant. embryoids readily formed shoots that were rooted on MS + 0.0luM NAA + 5% sucrose. Potential applications are discussed.
650 $aCitrus
650 $aCálcio
653 $aAlginate
653 $aAlginato
653 $aCalciun
653 $aEmbriogenese Somatic
653 $aEmbriogenese Somatica
653 $aProtoplast
653 $aProtoplastos
773 $tPlant Cell, Tissue and Organ Culture$gv.34, n.1, p.19-25, 1993.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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